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Comparative evaluation of linear and exponential amplification techniques for expression profiling at the single-cell level

BACKGROUND: Single-cell microarray expression profiling requires 10(8)-10(9)-fold amplification of the picogram amounts of total RNA typically found in eukaryotic cells. Several methods for RNA amplification are in general use, but little consideration has been given to the comparative analysis of t...

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Detalles Bibliográficos
Autores principales: Subkhankulova, Tatiana, Livesey, Frederick J
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557751/
https://www.ncbi.nlm.nih.gov/pubmed/16542485
http://dx.doi.org/10.1186/gb-2006-7-3-r18

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