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Induction of single chain tetracycline repressor requires the binding of two inducers

In this article we report the in vivo and in vitro characterization of single chain tetracycline repressor (scTetR) variants in Escherichia coli. ScTetR is genetically and proteolytically stable and exhibits the same regulatory properties as dimeric TetR in E.coli. Urea-dependent denaturation of scT...

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Detalles Bibliográficos
Autores principales: Kamionka, Annette, Majewski, Marius, Roth, Karin, Bertram, Ralph, Kraft, Christine, Hillen, Wolfgang
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557800/
https://www.ncbi.nlm.nih.gov/pubmed/16899452
http://dx.doi.org/10.1093/nar/gkl316