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Identification of small non-coding RNAs from mitochondria and chloroplasts
Small non-protein-coding RNAs (ncRNAs) have been identified in a wide spectrum of organisms ranging from bacteria to humans. In eukarya, systematic searches for ncRNAs have so far been restricted to the nuclear or cytosolic compartments of cells. Whether or not small stable non-coding RNA species al...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557801/ https://www.ncbi.nlm.nih.gov/pubmed/16899451 http://dx.doi.org/10.1093/nar/gkl448 |
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author | Lung, Birgit Zemann, Anja Madej, Monika J. Schuelke, Markus Techritz, Sandra Ruf, Stephanie Bock, Ralph Hüttenhofer, Alexander |
author_facet | Lung, Birgit Zemann, Anja Madej, Monika J. Schuelke, Markus Techritz, Sandra Ruf, Stephanie Bock, Ralph Hüttenhofer, Alexander |
author_sort | Lung, Birgit |
collection | PubMed |
description | Small non-protein-coding RNAs (ncRNAs) have been identified in a wide spectrum of organisms ranging from bacteria to humans. In eukarya, systematic searches for ncRNAs have so far been restricted to the nuclear or cytosolic compartments of cells. Whether or not small stable non-coding RNA species also exist in cell organelles, in addition to tRNAs or ribosomal RNAs, is unknown. We have thus generated cDNA libraries from size-selected mammalian mitochondrial RNA and plant chloroplast RNA and searched for small ncRNA species in these two types of DNA-containing cell organelles. In total, we have identified 18 novel candidates for organellar ncRNAs in these two cellular compartments and confirmed expression of six of them by northern blot analysis or RNase A protection assays. Most candidate ncRNA genes map to intergenic regions of the organellar genomes. As found previously in bacteria, the presumptive ancestors of present-day chloroplasts and mitochondria, we also observed examples of antisense ncRNAs that potentially could target organelle-encoded mRNAs. The structural features of the identified ncRNAs as well as their possible cellular functions are discussed. The absence from our libraries of abundant small RNA species that are not encoded by the organellar genomes suggests that the import of RNAs into cell organelles is of very limited significance or does not occur at all. |
format | Text |
id | pubmed-1557801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-15578012006-09-08 Identification of small non-coding RNAs from mitochondria and chloroplasts Lung, Birgit Zemann, Anja Madej, Monika J. Schuelke, Markus Techritz, Sandra Ruf, Stephanie Bock, Ralph Hüttenhofer, Alexander Nucleic Acids Res RNA Small non-protein-coding RNAs (ncRNAs) have been identified in a wide spectrum of organisms ranging from bacteria to humans. In eukarya, systematic searches for ncRNAs have so far been restricted to the nuclear or cytosolic compartments of cells. Whether or not small stable non-coding RNA species also exist in cell organelles, in addition to tRNAs or ribosomal RNAs, is unknown. We have thus generated cDNA libraries from size-selected mammalian mitochondrial RNA and plant chloroplast RNA and searched for small ncRNA species in these two types of DNA-containing cell organelles. In total, we have identified 18 novel candidates for organellar ncRNAs in these two cellular compartments and confirmed expression of six of them by northern blot analysis or RNase A protection assays. Most candidate ncRNA genes map to intergenic regions of the organellar genomes. As found previously in bacteria, the presumptive ancestors of present-day chloroplasts and mitochondria, we also observed examples of antisense ncRNAs that potentially could target organelle-encoded mRNAs. The structural features of the identified ncRNAs as well as their possible cellular functions are discussed. The absence from our libraries of abundant small RNA species that are not encoded by the organellar genomes suggests that the import of RNAs into cell organelles is of very limited significance or does not occur at all. Oxford University Press 2006 2006-08-09 /pmc/articles/PMC1557801/ /pubmed/16899451 http://dx.doi.org/10.1093/nar/gkl448 Text en © 2006 The Author(s). |
spellingShingle | RNA Lung, Birgit Zemann, Anja Madej, Monika J. Schuelke, Markus Techritz, Sandra Ruf, Stephanie Bock, Ralph Hüttenhofer, Alexander Identification of small non-coding RNAs from mitochondria and chloroplasts |
title | Identification of small non-coding RNAs from mitochondria and chloroplasts |
title_full | Identification of small non-coding RNAs from mitochondria and chloroplasts |
title_fullStr | Identification of small non-coding RNAs from mitochondria and chloroplasts |
title_full_unstemmed | Identification of small non-coding RNAs from mitochondria and chloroplasts |
title_short | Identification of small non-coding RNAs from mitochondria and chloroplasts |
title_sort | identification of small non-coding rnas from mitochondria and chloroplasts |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557801/ https://www.ncbi.nlm.nih.gov/pubmed/16899451 http://dx.doi.org/10.1093/nar/gkl448 |
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