Cargando…

Elongation complexes of Thermus thermophilus RNA polymerase that possess distinct translocation conformations

We have characterized elongation complexes (ECs) of RNA polymerase from the extremely thermophilic bacterium, Thermus thermophilus. We found that complexes assembled on nucleic acid scaffolds are transcriptionally competent at high temperature (50–80°C) and, depending upon the organization of the sc...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kashkina, Ekaterina, Anikin, Michael, Tahirov, Tahir H., Kochetkov, Sergei N., Vassylyev, Dmitry G., Temiakov, Dmitry
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557819/ https://www.ncbi.nlm.nih.gov/pubmed/16914440 http://dx.doi.org/10.1093/nar/gkl559

Ejemplares similares

Allosteric control of the RNA polymerase by the elongation factor RfaH
por: Svetlov, Vladimir, et al.
Publicado: (2007)

A novel intermediate in transcription initiation by human mitochondrial RNA polymerase
por: Morozov, Yaroslav I., et al.
Publicado: (2014)

Single molecule analysis of Thermus thermophilus SSB protein dynamics on single-stranded DNA
por: Zhang, Jichuan, et al.
Publicado: (2014)

Characterization of DNA polymerase X from Thermus thermophilus HB8 reveals the POLXc and PHP domains are both required for 3′–5′ exonuclease activity
por: Nakane, Shuhei, et al.
Publicado: (2009)

A novel dimerization motif in the C-terminal domain of the Thermus thermophilus DEAD box helicase Hera confers substantial flexibility(†)
por: Klostermeier, Dagmar, et al.
Publicado: (2009)

Pseudouridine at position 55 in tRNA controls the contents of other modified nucleotides for low-temperature adaptation in the extreme-thermophilic eubacterium Thermus thermophilus
por: Ishida, Kazuo, et al.
Publicado: (2011)

Helix–hairpin–helix protein MJ1434 from Methanocaldococcus jannaschii and EndoIV homologue TTC0482 from Thermus thermophilus HB27 do not process DNA uracil residues
por: Schomacher, Lars, et al.
Publicado: (2010)

N(7)-Methylguanine at position 46 (m(7)G46) in tRNA from Thermus thermophilus is required for cell viability at high temperatures through a tRNA modification network
por: Tomikawa, Chie, et al.
Publicado: (2010)

Active site opening and closure control translocation of multisubunit RNA polymerase
por: Malinen, Anssi M., et al.
Publicado: (2012)

Structural transitions in the transcription elongation complexes of bacterial RNA polymerase during σ-dependent pausing
por: Zhilina, Ekaterina, et al.
Publicado: (2012)

Spt4/5 stimulates transcription elongation through the RNA polymerase clamp coiled-coil motif
por: Hirtreiter, Angela, et al.
Publicado: (2010)

Human DNA polymerase θ possesses 5′-dRP lyase activity and functions in single-nucleotide base excision repair in vitro
por: Prasad, Rajendra, et al.
Publicado: (2009)

Nano positioning system reveals the course of upstream and nontemplate DNA within the RNA polymerase II elongation complex
por: Andrecka, Joanna, et al.
Publicado: (2009)

Multiple roles of the RNA polymerase β′ SW2 region in transcription initiation, promoter escape, and RNA elongation
por: Pupov, Danil, et al.
Publicado: (2010)

A nucleobase-binding pocket in a viral RNA-dependent RNA polymerase contributes to elongation complex stability
por: Shi, Wei, et al.
Publicado: (2020)

Nucleotide selection by the Y-family DNA polymerase Dpo4 involves template translocation and misalignment
por: Brenlla, Alfonso, et al.
Publicado: (2014)

Microhomology-mediated DNA strand annealing and elongation by human DNA polymerases λ and β on normal and repetitive DNA sequences
por: Crespan, Emmanuele, et al.
Publicado: (2012)

The methyltransferase domain of dengue virus protein NS5 ensures efficient RNA synthesis initiation and elongation by the polymerase domain
por: Potisopon, Supanee, et al.
Publicado: (2014)

Single-molecule analysis of 1D diffusion and transcription elongation of T7 RNA polymerase along individual stretched DNA molecules
por: Kim, Ji Hoon, et al.
Publicado: (2007)

The third restriction–modification system from Thermus aquaticus YT-1: solving the riddle of two TaqII specificities
por: Skowron, Piotr M., et al.
Publicado: (2017)

T7 RNA polymerase translocation is facilitated by a helix opening on the fingers domain that may also prevent backtracking
por: Da, Lin-Tai, et al.
Publicado: (2017)

Noncanonical prokaryotic X family DNA polymerases lack polymerase activity and act as exonucleases
por: Prostova, Maria, et al.
Publicado: (2022)

The role of catalytic and regulatory domains of human PrimPol in DNA binding and synthesis
por: Boldinova, Elizaveta O, et al.
Publicado: (2023)

RNA–DNA and DNA–DNA base-pairing at the upstream edge of the transcription bubble regulate translocation of RNA polymerase and transcription rate
por: KIreeva, Maria, et al.
Publicado: (2018)

Acetylation and phosphorylation of human TFAM regulate TFAM–DNA interactions via contrasting mechanisms
por: King, Graeme A, et al.
Publicado: (2018)

Thiostrepton inhibits stable 70S ribosome binding and ribosome-dependent GTPase activation of elongation factor G and elongation factor 4
por: Walter, Justin D., et al.
Publicado: (2012)

Substrate conformational dynamics facilitate structure-specific recognition of gapped DNA by DNA polymerase
por: Craggs, Timothy D, et al.
Publicado: (2019)

A non-canonical multisubunit RNA polymerase encoded by a giant bacteriophage
por: Yakunina, Maria, et al.
Publicado: (2015)

Engineering the elongation factor Tu for efficient selenoprotein synthesis
por: Haruna, Ken-ichi, et al.
Publicado: (2014)

Dynamics of human replication factors in the elongation phase of DNA replication
por: Masuda, Yuji, et al.
Publicado: (2007)

The DNA polymerase III holoenzyme contains γ and is not a trimeric polymerase
por: Dohrmann, Paul R., et al.
Publicado: (2016)

Plant organellar DNA polymerases are replicative and translesion DNA synthesis polymerases
por: Baruch-Torres, Noe, et al.
Publicado: (2017)

Alteration in the cavity size adjacent to the active site of RB69 DNA polymerase changes its conformational dynamics
por: Xia, Shuangluo, et al.
Publicado: (2013)

RNA polymerase clamp conformational dynamics: long-lived states and modulation by crowding, cations, and nonspecific DNA binding
por: Mazumder, Abhishek, et al.
Publicado: (2021)

A novel three-unit tRNA splicing endonuclease found in ultrasmall Archaea possesses broad substrate specificity
por: Fujishima, Kosuke, et al.
Publicado: (2011)

A ‘new lease of life’: FnCpf1 possesses DNA cleavage activity for genome editing in human cells
por: Tu, Mengjun, et al.
Publicado: (2017)

Allosteric couplings upon binding of RfaH to transcription elongation complexes
por: Molina, José Alejandro, et al.
Publicado: (2022)

DNA polymerase proofreading: active site switching catalyzed by the bacteriophage T4 DNA polymerase
por: Fidalgo da Silva, Elizabeth, et al.
Publicado: (2007)

Discovery of an essential nucleotidylating activity associated with a newly delineated conserved domain in the RNA polymerase-containing protein of all nidoviruses
por: Lehmann, Kathleen C., et al.
Publicado: (2015)

A non-canonical multisubunit RNA polymerase encoded by the AR9 phage recognizes the template strand of its uracil-containing promoters
por: Sokolova, Maria, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_dh944lpp1ug7tmge9bp2hvdat5