Isolation of a single-stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as zeta crystallin

A single-stranded DNA (ssDNA)-binding protein (SSB) that binds to specific upstream sequences of alcohol oxidase (AOX1) promoter of the methylotrophic yeast Pichia pastoris has been isolated and identified as zeta crystallin (ZTA1). The cDNA encoding P.pastoris ZTA1 (PpZTA1) was cloned into an Esche...

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Detalles Bibliográficos
Autores principales: Kranthi, Balla Venkata, Balasubramanian, Natarajan, Rangarajan, Pundi N.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Molecular Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557824/
https://www.ncbi.nlm.nih.gov/pubmed/16914438
http://dx.doi.org/10.1093/nar/gkl577
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author Kranthi, Balla Venkata
Balasubramanian, Natarajan
Rangarajan, Pundi N.
author_facet Kranthi, Balla Venkata
Balasubramanian, Natarajan
Rangarajan, Pundi N.
author_sort Kranthi, Balla Venkata
collection PubMed
description A single-stranded DNA (ssDNA)-binding protein (SSB) that binds to specific upstream sequences of alcohol oxidase (AOX1) promoter of the methylotrophic yeast Pichia pastoris has been isolated and identified as zeta crystallin (ZTA1). The cDNA encoding P.pastoris ZTA1 (PpZTA1) was cloned into an Escherichia coli expression vector, the recombinant PpZTA1 was expressed and purified from E.coli cell lysates. The DNA-binding properties of recombinant PpZTA1 are identical to those of the SSB present in P.pastoris cell lysates. PpZTA1 binds to ssDNA sequences >24 nt and its DNA-binding activity is abolished by NADPH. This is the first report on the characterization of DNA-binding properties of a yeast ZTA1.
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spelling pubmed-15578242006-09-08 Isolation of a single-stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as zeta crystallin Kranthi, Balla Venkata Balasubramanian, Natarajan Rangarajan, Pundi N. Nucleic Acids Res Molecular Biology A single-stranded DNA (ssDNA)-binding protein (SSB) that binds to specific upstream sequences of alcohol oxidase (AOX1) promoter of the methylotrophic yeast Pichia pastoris has been isolated and identified as zeta crystallin (ZTA1). The cDNA encoding P.pastoris ZTA1 (PpZTA1) was cloned into an Escherichia coli expression vector, the recombinant PpZTA1 was expressed and purified from E.coli cell lysates. The DNA-binding properties of recombinant PpZTA1 are identical to those of the SSB present in P.pastoris cell lysates. PpZTA1 binds to ssDNA sequences >24 nt and its DNA-binding activity is abolished by NADPH. This is the first report on the characterization of DNA-binding properties of a yeast ZTA1. Oxford University Press 2006 2006-08-16 /pmc/articles/PMC1557824/ /pubmed/16914438 http://dx.doi.org/10.1093/nar/gkl577 Text en © 2006 The Author(s).
spellingShingle Molecular Biology
Kranthi, Balla Venkata
Balasubramanian, Natarajan
Rangarajan, Pundi N.
Isolation of a single-stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as zeta crystallin
title Isolation of a single-stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as zeta crystallin
title_full Isolation of a single-stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as zeta crystallin
title_fullStr Isolation of a single-stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as zeta crystallin
title_full_unstemmed Isolation of a single-stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as zeta crystallin
title_short Isolation of a single-stranded DNA-binding protein from the methylotrophic yeast, Pichia pastoris and its identification as zeta crystallin
title_sort isolation of a single-stranded dna-binding protein from the methylotrophic yeast, pichia pastoris and its identification as zeta crystallin
topic Molecular Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557824/
https://www.ncbi.nlm.nih.gov/pubmed/16914438
http://dx.doi.org/10.1093/nar/gkl577
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AT balasubramaniannatarajan isolationofasinglestrandeddnabindingproteinfromthemethylotrophicyeastpichiapastorisanditsidentificationaszetacrystallin
AT rangarajanpundin isolationofasinglestrandeddnabindingproteinfromthemethylotrophicyeastpichiapastorisanditsidentificationaszetacrystallin

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