Cargando…
Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus
BACKGROUND: The T(Wis )mutant allele of the Brachyury, or T, gene was created by insertion of an endogenous retrovirus-like early transposon (ETn) element into the exon 7 splice donor consensus sequence of the 8 exon T locus. While the developmental consequences of this disruption have been well cha...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2003
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC155784/ https://www.ncbi.nlm.nih.gov/pubmed/12702217 http://dx.doi.org/10.1186/1471-2164-4-14 |
_version_ | 1782120770333310976 |
---|---|
author | Goldin, Sarah N Papaioannou, Virginia E |
author_facet | Goldin, Sarah N Papaioannou, Virginia E |
author_sort | Goldin, Sarah N |
collection | PubMed |
description | BACKGROUND: The T(Wis )mutant allele of the Brachyury, or T, gene was created by insertion of an endogenous retrovirus-like early transposon (ETn) element into the exon 7 splice donor consensus sequence of the 8 exon T locus. While the developmental consequences of this disruption have been well characterized, the molecular consequences have not been previously investigated, and it has been assumed that the insertion results in a truncated protein. This study sought to further characterize the mutant T(Wis )allele by investigating the nature of the transcripts produced by insertion of this transposable element. RESULTS: Using an RT-PCR based approach, we have shown that at least 8 different mutant transcripts are produced from the T(Wis )allele. All T(Wis )transcripts bypass the mutated exon 7 splice donor site, such that wild type T transcripts are not produced from the T(Wis )allele. CONCLUSIONS: This result shows an unsuspected misregulation of RNA splicing caused by insertion of a transposable element, that could have more widespread consequences in the genome. |
format | Text |
id | pubmed-155784 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-1557842003-05-21 Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus Goldin, Sarah N Papaioannou, Virginia E BMC Genomics Research Article BACKGROUND: The T(Wis )mutant allele of the Brachyury, or T, gene was created by insertion of an endogenous retrovirus-like early transposon (ETn) element into the exon 7 splice donor consensus sequence of the 8 exon T locus. While the developmental consequences of this disruption have been well characterized, the molecular consequences have not been previously investigated, and it has been assumed that the insertion results in a truncated protein. This study sought to further characterize the mutant T(Wis )allele by investigating the nature of the transcripts produced by insertion of this transposable element. RESULTS: Using an RT-PCR based approach, we have shown that at least 8 different mutant transcripts are produced from the T(Wis )allele. All T(Wis )transcripts bypass the mutated exon 7 splice donor site, such that wild type T transcripts are not produced from the T(Wis )allele. CONCLUSIONS: This result shows an unsuspected misregulation of RNA splicing caused by insertion of a transposable element, that could have more widespread consequences in the genome. BioMed Central 2003-04-17 /pmc/articles/PMC155784/ /pubmed/12702217 http://dx.doi.org/10.1186/1471-2164-4-14 Text en Copyright © 2003 Goldin and Papaioannou; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Research Article Goldin, Sarah N Papaioannou, Virginia E Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus |
title | Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus |
title_full | Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus |
title_fullStr | Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus |
title_full_unstemmed | Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus |
title_short | Unusual misregulation of RNA splicing caused by insertion of a transposable element into the T (Brachyury) locus |
title_sort | unusual misregulation of rna splicing caused by insertion of a transposable element into the t (brachyury) locus |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC155784/ https://www.ncbi.nlm.nih.gov/pubmed/12702217 http://dx.doi.org/10.1186/1471-2164-4-14 |
work_keys_str_mv | AT goldinsarahn unusualmisregulationofrnasplicingcausedbyinsertionofatransposableelementintothetbrachyurylocus AT papaioannouvirginiae unusualmisregulationofrnasplicingcausedbyinsertionofatransposableelementintothetbrachyurylocus |