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Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells
BACKGROUND: There is a growing interest in cord blood as a source of primitive stem cells with the capacity for multilineage differentiation. Pure cell fractions are needed for the characterization and in vitro expansion of stem cells as well as for their use in preclinical research. However, enrich...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557844/ https://www.ncbi.nlm.nih.gov/pubmed/16882340 http://dx.doi.org/10.1186/1471-2121-7-30 |
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author | Kekarainen, Tuija Mannelin, Sirkka Laine, Jarmo Jaatinen, Taina |
author_facet | Kekarainen, Tuija Mannelin, Sirkka Laine, Jarmo Jaatinen, Taina |
author_sort | Kekarainen, Tuija |
collection | PubMed |
description | BACKGROUND: There is a growing interest in cord blood as a source of primitive stem cells with the capacity for multilineage differentiation. Pure cell fractions are needed for the characterization and in vitro expansion of stem cells as well as for their use in preclinical research. However, enrichment of stem cells is challenging due to the lack of stem cell-specific markers and gentle protocols for the isolation of highly pure stem cell fractions. Protocols developed for the enrichment of peripheral blood-derived stem cells have been found to be suboptimal for cord blood. RESULTS: In this study, immunomagnetic cell sorting protocols to purify CD34+, CD133+ and Lin- cells from fresh and cryopreserved cord blood were optimized. Reproducible purities of up to 97% were reached. The selected cells were highly viable having substantial colony-forming potential. CONCLUSION: The optimized protocols enable rapid enrichment of highly pure hematopoietic stem cells from both fresh and cryopreserved cord blood. |
format | Text |
id | pubmed-1557844 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-15578442006-09-01 Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells Kekarainen, Tuija Mannelin, Sirkka Laine, Jarmo Jaatinen, Taina BMC Cell Biol Methodology Article BACKGROUND: There is a growing interest in cord blood as a source of primitive stem cells with the capacity for multilineage differentiation. Pure cell fractions are needed for the characterization and in vitro expansion of stem cells as well as for their use in preclinical research. However, enrichment of stem cells is challenging due to the lack of stem cell-specific markers and gentle protocols for the isolation of highly pure stem cell fractions. Protocols developed for the enrichment of peripheral blood-derived stem cells have been found to be suboptimal for cord blood. RESULTS: In this study, immunomagnetic cell sorting protocols to purify CD34+, CD133+ and Lin- cells from fresh and cryopreserved cord blood were optimized. Reproducible purities of up to 97% were reached. The selected cells were highly viable having substantial colony-forming potential. CONCLUSION: The optimized protocols enable rapid enrichment of highly pure hematopoietic stem cells from both fresh and cryopreserved cord blood. BioMed Central 2006-08-01 /pmc/articles/PMC1557844/ /pubmed/16882340 http://dx.doi.org/10.1186/1471-2121-7-30 Text en Copyright © 2006 Kekarainen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Methodology Article Kekarainen, Tuija Mannelin, Sirkka Laine, Jarmo Jaatinen, Taina Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells |
title | Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells |
title_full | Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells |
title_fullStr | Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells |
title_full_unstemmed | Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells |
title_short | Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells |
title_sort | optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1557844/ https://www.ncbi.nlm.nih.gov/pubmed/16882340 http://dx.doi.org/10.1186/1471-2121-7-30 |
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