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Generation of RNAi Libraries for High-Throughput Screens

The completion of the genome sequencing for several organisms has created a great demand for genomic tools that can systematically analyze the growing wealth of data. In contrast to the classical reverse genetics approach of creating specific knockout cell lines or animals that is time-consuming and...

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Detalles Bibliográficos
Autores principales: Clark, Julie, Ding, Sheng
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559919/
https://www.ncbi.nlm.nih.gov/pubmed/17057364
http://dx.doi.org/10.1155/JBB/2006/45716
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author Clark, Julie
Ding, Sheng
author_facet Clark, Julie
Ding, Sheng
author_sort Clark, Julie
collection PubMed
description The completion of the genome sequencing for several organisms has created a great demand for genomic tools that can systematically analyze the growing wealth of data. In contrast to the classical reverse genetics approach of creating specific knockout cell lines or animals that is time-consuming and expensive, RNA-mediated interference (RNAi) has emerged as a fast, simple, and cost-effective technique for gene knockdown in large scale. Since its discovery as a gene silencing response to double-stranded RNA (dsRNA) with homology to endogenous genes in Caenorhabditis elegans (C elegans), RNAi technology has been adapted to various high-throughput screens (HTS) for genome-wide loss-of-function (LOF) analysis. Biochemical insights into the endogenous mechanism of RNAi have led to advances in RNAi methodology including RNAi molecule synthesis, delivery, and sequence design. In this article, we will briefly review these various RNAi library designs and discuss the benefits and drawbacks of each library strategy.
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spelling pubmed-15599192006-10-10 Generation of RNAi Libraries for High-Throughput Screens Clark, Julie Ding, Sheng J Biomed Biotechnol Review Article The completion of the genome sequencing for several organisms has created a great demand for genomic tools that can systematically analyze the growing wealth of data. In contrast to the classical reverse genetics approach of creating specific knockout cell lines or animals that is time-consuming and expensive, RNA-mediated interference (RNAi) has emerged as a fast, simple, and cost-effective technique for gene knockdown in large scale. Since its discovery as a gene silencing response to double-stranded RNA (dsRNA) with homology to endogenous genes in Caenorhabditis elegans (C elegans), RNAi technology has been adapted to various high-throughput screens (HTS) for genome-wide loss-of-function (LOF) analysis. Biochemical insights into the endogenous mechanism of RNAi have led to advances in RNAi methodology including RNAi molecule synthesis, delivery, and sequence design. In this article, we will briefly review these various RNAi library designs and discuss the benefits and drawbacks of each library strategy. Hindawi Publishing Corporation 2006 2006-06-08 /pmc/articles/PMC1559919/ /pubmed/17057364 http://dx.doi.org/10.1155/JBB/2006/45716 Text en Copyright © 2006 J. Clark and S. Ding. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Clark, Julie
Ding, Sheng
Generation of RNAi Libraries for High-Throughput Screens
title Generation of RNAi Libraries for High-Throughput Screens
title_full Generation of RNAi Libraries for High-Throughput Screens
title_fullStr Generation of RNAi Libraries for High-Throughput Screens
title_full_unstemmed Generation of RNAi Libraries for High-Throughput Screens
title_short Generation of RNAi Libraries for High-Throughput Screens
title_sort generation of rnai libraries for high-throughput screens
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559919/
https://www.ncbi.nlm.nih.gov/pubmed/17057364
http://dx.doi.org/10.1155/JBB/2006/45716
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