Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi

Short interfering RNAs (siRNAs) are widely used to bring about RNA interference (RNAi) in mammalian cells. Numerous siRNAs may be designed for any target gene though most of which would be incapable of efficiently inducing mammalian RNAi. Certain highly functional siRNAs designed for knockout of a p...

Descripción completa

Detalles Bibliográficos
Autores principales: Ui-Tei, Kumiko, Naito, Yuki, Saigo, Kaoru
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2006
Materias:
Review Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559925/
https://www.ncbi.nlm.nih.gov/pubmed/17057367
http://dx.doi.org/10.1155/JBB/2006/65052
_version_ 1782129461002502144
author Ui-Tei, Kumiko
Naito, Yuki
Saigo, Kaoru
author_facet Ui-Tei, Kumiko
Naito, Yuki
Saigo, Kaoru
author_sort Ui-Tei, Kumiko
collection PubMed
description Short interfering RNAs (siRNAs) are widely used to bring about RNA interference (RNAi) in mammalian cells. Numerous siRNAs may be designed for any target gene though most of which would be incapable of efficiently inducing mammalian RNAi. Certain highly functional siRNAs designed for knockout of a particular gene may render unrelated endogenous genes nonfunctional. These major bottlenecks should be properly eliminated when RNAi technologies are employed for any experiment in mammalian functional genomics. This paper thus presents essential notes and findings regarding the proper choice of siRNA-sequence selection algorithms and web-based online software systems.
format Text
id pubmed-1559925
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-15599252006-10-10 Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi Ui-Tei, Kumiko Naito, Yuki Saigo, Kaoru J Biomed Biotechnol Review Article Short interfering RNAs (siRNAs) are widely used to bring about RNA interference (RNAi) in mammalian cells. Numerous siRNAs may be designed for any target gene though most of which would be incapable of efficiently inducing mammalian RNAi. Certain highly functional siRNAs designed for knockout of a particular gene may render unrelated endogenous genes nonfunctional. These major bottlenecks should be properly eliminated when RNAi technologies are employed for any experiment in mammalian functional genomics. This paper thus presents essential notes and findings regarding the proper choice of siRNA-sequence selection algorithms and web-based online software systems. Hindawi Publishing Corporation 2006 2006-07-03 /pmc/articles/PMC1559925/ /pubmed/17057367 http://dx.doi.org/10.1155/JBB/2006/65052 Text en Copyright © 2006 Kumiko Ui-Tei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review Article
Ui-Tei, Kumiko
Naito, Yuki
Saigo, Kaoru
Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi
title Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi
title_full Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi
title_fullStr Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi
title_full_unstemmed Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi
title_short Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi
title_sort essential notes regarding the design of functional sirnas for efficient mammalian rnai
topic Review Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559925/
https://www.ncbi.nlm.nih.gov/pubmed/17057367
http://dx.doi.org/10.1155/JBB/2006/65052
work_keys_str_mv AT uiteikumiko essentialnotesregardingthedesignoffunctionalsirnasforefficientmammalianrnai
AT naitoyuki essentialnotesregardingthedesignoffunctionalsirnasforefficientmammalianrnai
AT saigokaoru essentialnotesregardingthedesignoffunctionalsirnasforefficientmammalianrnai

Ejemplares similares