Cargando…
Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi
Short interfering RNAs (siRNAs) are widely used to bring about RNA interference (RNAi) in mammalian cells. Numerous siRNAs may be designed for any target gene though most of which would be incapable of efficiently inducing mammalian RNAi. Certain highly functional siRNAs designed for knockout of a p...
Autores principales: | Ui-Tei, Kumiko, Naito, Yuki, Saigo, Kaoru |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2006
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1559925/ https://www.ncbi.nlm.nih.gov/pubmed/17057367 http://dx.doi.org/10.1155/JBB/2006/65052 |
Ejemplares similares
-
siVirus: web-based antiviral siRNA design software for highly divergent viral sequences
por: Naito, Yuki, et al.
Publicado: (2006) -
Thermodynamic stability and Watson–Crick base pairing in the seed duplex are major determinants of the efficiency of the siRNA-based off-target effect
por: Ui-Tei, Kumiko, et al.
Publicado: (2008) -
Optimal choice of functional and off-target effect-reduced siRNAs for RNAi therapeutics
por: Ui-Tei, Kumiko
Publicado: (2013) -
Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect
por: Ui-Tei, Kumiko, et al.
Publicado: (2008) -
Optimal design and validation of antiviral siRNA for targeting HIV-1
por: Naito, Yuki, et al.
Publicado: (2007)