Combined single-molecule force and fluorescence measurements for biology

Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes ca...

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Detalles Bibliográficos
Autores principales: Wallace, Mark I, Molloy, Justin E, Trentham, David R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Minireview
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC156595/
https://www.ncbi.nlm.nih.gov/pubmed/12733995
http://dx.doi.org/10.1186/1475-4924-2-4
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author Wallace, Mark I
Molloy, Justin E
Trentham, David R
author_facet Wallace, Mark I
Molloy, Justin E
Trentham, David R
author_sort Wallace, Mark I
collection PubMed
description Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes can now be studied with nanometer precision and millisecond time resolution.
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spelling pubmed-1565952003-06-05 Combined single-molecule force and fluorescence measurements for biology Wallace, Mark I Molloy, Justin E Trentham, David R J Biol Minireview Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes can now be studied with nanometer precision and millisecond time resolution. BioMed Central 2003 2003-04-14 /pmc/articles/PMC156595/ /pubmed/12733995 http://dx.doi.org/10.1186/1475-4924-2-4 Text en Copyright © 2003 BioMed Central Ltd
spellingShingle Minireview
Wallace, Mark I
Molloy, Justin E
Trentham, David R
Combined single-molecule force and fluorescence measurements for biology
title Combined single-molecule force and fluorescence measurements for biology
title_full Combined single-molecule force and fluorescence measurements for biology
title_fullStr Combined single-molecule force and fluorescence measurements for biology
title_full_unstemmed Combined single-molecule force and fluorescence measurements for biology
title_short Combined single-molecule force and fluorescence measurements for biology
title_sort combined single-molecule force and fluorescence measurements for biology
topic Minireview
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC156595/
https://www.ncbi.nlm.nih.gov/pubmed/12733995
http://dx.doi.org/10.1186/1475-4924-2-4
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