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A general cloning system to selectively isolate any eukaryotic or prokaryotic genomic region in yeast
BACKGROUND: Transformation-associated recombination (TAR) cloning in yeast is a unique method for selective isolation of large chromosomal fragments or entire genes from complex genomes. The technique involves homologous recombination, during yeast spheroplast transformation, between genomic DNA and...
Autores principales: | Noskov, Vladimir N, Kouprina, Natalay, Leem, Sun-Hee, Ouspenski, Ilia, Barrett, J Carl, Larionov, Vladimir |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2003
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC156606/ https://www.ncbi.nlm.nih.gov/pubmed/12720573 http://dx.doi.org/10.1186/1471-2164-4-16 |
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