Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.

Collaborative studies were performed to develop a functional assay for fish-killing activity produced by Pfiesteria piscicida. Eight cell lines were used to screen organic fractions and residual water fraction by using a 3-[4, 5-dimethylthiazol-(2-4)]-diphenyltetrazolium bromide cytotoxicity assay....

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Detalles Bibliográficos
Autores principales: Fairey, E R, Edmunds, J S, Deamer-Melia, N J, Glasgow, H, Johnson, F M, Moeller, P R, Burkholder, J M, Ramsdell, J S
Formato: Texto
Lenguaje:English
Publicado: 1999
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1566442/
https://www.ncbi.nlm.nih.gov/pubmed/10464070
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author Fairey, E R
Edmunds, J S
Deamer-Melia, N J
Glasgow, H
Johnson, F M
Moeller, P R
Burkholder, J M
Ramsdell, J S
author_facet Fairey, E R
Edmunds, J S
Deamer-Melia, N J
Glasgow, H
Johnson, F M
Moeller, P R
Burkholder, J M
Ramsdell, J S
author_sort Fairey, E R
collection PubMed
description Collaborative studies were performed to develop a functional assay for fish-killing activity produced by Pfiesteria piscicida. Eight cell lines were used to screen organic fractions and residual water fraction by using a 3-[4, 5-dimethylthiazol-(2-4)]-diphenyltetrazolium bromide cytotoxicity assay. Diethyl ether and a residual water fraction were cytotoxic to several cell lines including rat pituitary (GH(4)C(1)) cells. Residual water as well as preextracted culture water containing P. piscicida cells induced c-fos-luciferase expressed in GH(4)C(1) cells with a rapid time course of induction and sensitive detection. The reporter gene assay detected activity in toxic isolates of P. piscicida from several North Carolina estuaries in 1997 and 1998 and may also be suitable for detecting toxic activity in human and animal serum.
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spelling pubmed-15664422006-09-19 Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida. Fairey, E R Edmunds, J S Deamer-Melia, N J Glasgow, H Johnson, F M Moeller, P R Burkholder, J M Ramsdell, J S Environ Health Perspect Research Article Collaborative studies were performed to develop a functional assay for fish-killing activity produced by Pfiesteria piscicida. Eight cell lines were used to screen organic fractions and residual water fraction by using a 3-[4, 5-dimethylthiazol-(2-4)]-diphenyltetrazolium bromide cytotoxicity assay. Diethyl ether and a residual water fraction were cytotoxic to several cell lines including rat pituitary (GH(4)C(1)) cells. Residual water as well as preextracted culture water containing P. piscicida cells induced c-fos-luciferase expressed in GH(4)C(1) cells with a rapid time course of induction and sensitive detection. The reporter gene assay detected activity in toxic isolates of P. piscicida from several North Carolina estuaries in 1997 and 1998 and may also be suitable for detecting toxic activity in human and animal serum. 1999-09 /pmc/articles/PMC1566442/ /pubmed/10464070 Text en
spellingShingle Research Article
Fairey, E R
Edmunds, J S
Deamer-Melia, N J
Glasgow, H
Johnson, F M
Moeller, P R
Burkholder, J M
Ramsdell, J S
Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.
title Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.
title_full Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.
title_fullStr Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.
title_full_unstemmed Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.
title_short Reporter gene assay for fish-killing activity produced by Pfiesteria piscicida.
title_sort reporter gene assay for fish-killing activity produced by pfiesteria piscicida.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1566442/
https://www.ncbi.nlm.nih.gov/pubmed/10464070
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