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Interactive effects between trichloroethylene and pesticides at metabolic and genetic level in mice.

A combined cytogeneticurine metabolite analysis approach was used to assess potential interactive effects between Fenarimol (FN), a fungicide, and trichloroethylene (TRI), a halogenated solvent. FN was demonstrated to selectively induce P450-2B1 isoforms in different organs of treated mice. Since th...

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Autores principales: Hrelia, P, Maffei, F, Vigagni, F, Fimognari, C, Flori, P, Stanzani, R, Cantelli Forti, G
Formato: Texto
Lenguaje:English
Publicado: 1994
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1566801/
https://www.ncbi.nlm.nih.gov/pubmed/7698080
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author Hrelia, P
Maffei, F
Vigagni, F
Fimognari, C
Flori, P
Stanzani, R
Cantelli Forti, G
author_facet Hrelia, P
Maffei, F
Vigagni, F
Fimognari, C
Flori, P
Stanzani, R
Cantelli Forti, G
author_sort Hrelia, P
collection PubMed
description A combined cytogeneticurine metabolite analysis approach was used to assess potential interactive effects between Fenarimol (FN), a fungicide, and trichloroethylene (TRI), a halogenated solvent. FN was demonstrated to selectively induce P450-2B1 isoforms in different organs of treated mice. Since the rate of metabolism and the stereospecificity of metabolism are dependent on the types and amount of P450s available, FN might drastically alter the metabolic activation of a precarcinogen, such as TRI, and its toxicological consequences. Male CD1 mice were divided into untreated, vehicle control, and experimental groups. Animals of the latter groups were treated ip with 150 mg/kg bw FN in corn oil, 457 mg/kg bw TRI in corn oil, TRI plus FN separated by different time intervals. Bone marrow cells were harvested for determination of micronuclei (MN) frequencies in polychromatic erythrocytes (PCE). The presence of the known metabolite of TRI, trichloroethanol (TCE), was quantitated in collected urine by gas chromatography using an electron-capture detector. Linear regression analysis shows that MN frequency by TRI is correlated with TCE concentration in urine. Observed potentiation of genotoxicity of TRI by FN pretreatment (1 hr before TRI treatment) apparently reflects changes in the spectra of enzymes involved in TRI metabolism, and altered toxicokinetic, as witnessed by the 20% difference in TCE excretion from combined treated mice. However, no increased genetic or metabolic effects were observed when FN was administered 3 hr before TRI. No significant interactive effects were observed at a genetic level when FN was administered 1 hr and 3 hr after TRI whereas a 33 to 47% loss in TCE excretion was recorded.(ABSTRACT TRUNCATED AT 250 WORDS)
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spelling pubmed-15668012006-09-19 Interactive effects between trichloroethylene and pesticides at metabolic and genetic level in mice. Hrelia, P Maffei, F Vigagni, F Fimognari, C Flori, P Stanzani, R Cantelli Forti, G Environ Health Perspect Research Article A combined cytogeneticurine metabolite analysis approach was used to assess potential interactive effects between Fenarimol (FN), a fungicide, and trichloroethylene (TRI), a halogenated solvent. FN was demonstrated to selectively induce P450-2B1 isoforms in different organs of treated mice. Since the rate of metabolism and the stereospecificity of metabolism are dependent on the types and amount of P450s available, FN might drastically alter the metabolic activation of a precarcinogen, such as TRI, and its toxicological consequences. Male CD1 mice were divided into untreated, vehicle control, and experimental groups. Animals of the latter groups were treated ip with 150 mg/kg bw FN in corn oil, 457 mg/kg bw TRI in corn oil, TRI plus FN separated by different time intervals. Bone marrow cells were harvested for determination of micronuclei (MN) frequencies in polychromatic erythrocytes (PCE). The presence of the known metabolite of TRI, trichloroethanol (TCE), was quantitated in collected urine by gas chromatography using an electron-capture detector. Linear regression analysis shows that MN frequency by TRI is correlated with TCE concentration in urine. Observed potentiation of genotoxicity of TRI by FN pretreatment (1 hr before TRI treatment) apparently reflects changes in the spectra of enzymes involved in TRI metabolism, and altered toxicokinetic, as witnessed by the 20% difference in TCE excretion from combined treated mice. However, no increased genetic or metabolic effects were observed when FN was administered 3 hr before TRI. No significant interactive effects were observed at a genetic level when FN was administered 1 hr and 3 hr after TRI whereas a 33 to 47% loss in TCE excretion was recorded.(ABSTRACT TRUNCATED AT 250 WORDS) 1994-11 /pmc/articles/PMC1566801/ /pubmed/7698080 Text en
spellingShingle Research Article
Hrelia, P
Maffei, F
Vigagni, F
Fimognari, C
Flori, P
Stanzani, R
Cantelli Forti, G
Interactive effects between trichloroethylene and pesticides at metabolic and genetic level in mice.
title Interactive effects between trichloroethylene and pesticides at metabolic and genetic level in mice.
title_full Interactive effects between trichloroethylene and pesticides at metabolic and genetic level in mice.
title_fullStr Interactive effects between trichloroethylene and pesticides at metabolic and genetic level in mice.
title_full_unstemmed Interactive effects between trichloroethylene and pesticides at metabolic and genetic level in mice.
title_short Interactive effects between trichloroethylene and pesticides at metabolic and genetic level in mice.
title_sort interactive effects between trichloroethylene and pesticides at metabolic and genetic level in mice.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1566801/
https://www.ncbi.nlm.nih.gov/pubmed/7698080
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