DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines.

The induction of cytotoxicity and DNA single-strand breaks by chromium(VI), cadmium(II), and mercury(II) were compared in H2O2-resistant Chinese hamster ovary (CHO(R)) cells and parental (CHO(P)) cells. Using a colony-forming assay, CHO(R) cells were found to be significantly more resistant than CHO...

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Autores principales: Tsuzuki, K, Sugiyama, M, Haramaki, N
Formato: Texto
Lenguaje:English
Publicado: 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567393/
https://www.ncbi.nlm.nih.gov/pubmed/7843132
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author Tsuzuki, K
Sugiyama, M
Haramaki, N
author_facet Tsuzuki, K
Sugiyama, M
Haramaki, N
author_sort Tsuzuki, K
collection PubMed
description The induction of cytotoxicity and DNA single-strand breaks by chromium(VI), cadmium(II), and mercury(II) were compared in H2O2-resistant Chinese hamster ovary (CHO(R)) cells and parental (CHO(P)) cells. Using a colony-forming assay, CHO(R) cells were found to be significantly more resistant than CHO(P) cells to the cytotoxicity caused by CdCl2 and HgCl2, but not to that caused by Na2CrO4. However, the DNA single-strand breaks produced by each of these metals were significantly lower in the CHO(R) cells. With respect to chromium reduction, the level of chromium(V) in CHO(R) cells was decreased. The role of intracellular active oxygen in the heavy metal-induced DNA damage and cytotoxicity is discussed.
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spelling pubmed-15673932006-09-19 DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines. Tsuzuki, K Sugiyama, M Haramaki, N Environ Health Perspect Research Article The induction of cytotoxicity and DNA single-strand breaks by chromium(VI), cadmium(II), and mercury(II) were compared in H2O2-resistant Chinese hamster ovary (CHO(R)) cells and parental (CHO(P)) cells. Using a colony-forming assay, CHO(R) cells were found to be significantly more resistant than CHO(P) cells to the cytotoxicity caused by CdCl2 and HgCl2, but not to that caused by Na2CrO4. However, the DNA single-strand breaks produced by each of these metals were significantly lower in the CHO(R) cells. With respect to chromium reduction, the level of chromium(V) in CHO(R) cells was decreased. The role of intracellular active oxygen in the heavy metal-induced DNA damage and cytotoxicity is discussed. 1994-09 /pmc/articles/PMC1567393/ /pubmed/7843132 Text en
spellingShingle Research Article
Tsuzuki, K
Sugiyama, M
Haramaki, N
DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines.
title DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines.
title_full DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines.
title_fullStr DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines.
title_full_unstemmed DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines.
title_short DNA single-strand breaks and cytotoxicity induced by chromate(VI), cadmium(II), and mercury(II) in hydrogen peroxide-resistant cell lines.
title_sort dna single-strand breaks and cytotoxicity induced by chromate(vi), cadmium(ii), and mercury(ii) in hydrogen peroxide-resistant cell lines.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567393/
https://www.ncbi.nlm.nih.gov/pubmed/7843132
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AT haramakin dnasinglestrandbreaksandcytotoxicityinducedbychromatevicadmiumiiandmercuryiiinhydrogenperoxideresistantcelllines

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