Monitoring metal concentrations in tissues and single cells using ultramicrosensors.

Intercellular and extracellular metal concentrations were measured using carbon fiber ultramicrosensors plated with mercury or with polymeric porphyrinic p-type semiconductors. Concentrations of unbound nickel and lead ions were studied within individual BC3H-1 myocytes, and H4-11-C3 rat hepatoma ce...

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Detalles Bibliográficos
Autores principales: Malinski, T, Grunfeld, S, Taha, Z, Tomboulian, P
Formato: Texto
Lenguaje:English
Publicado: 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567395/
https://www.ncbi.nlm.nih.gov/pubmed/7843090
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author Malinski, T
Grunfeld, S
Taha, Z
Tomboulian, P
author_facet Malinski, T
Grunfeld, S
Taha, Z
Tomboulian, P
author_sort Malinski, T
collection PubMed
description Intercellular and extracellular metal concentrations were measured using carbon fiber ultramicrosensors plated with mercury or with polymeric porphyrinic p-type semiconductors. Concentrations of unbound nickel and lead ions were studied within individual BC3H-1 myocytes, and H4-11-C3 rat hepatoma cells. Unbound ions are predominantly solvated inorganic ions not coordinated to biological cellular components. Fabrication of ultramicrosensors appropriate for the cells under investigation is described, including procedures for sharpening and waxing the microsensors in order to control the shape, area, and dimensions of the electroactive surface. Metal ion movement through cell membranes and intracellular ion diffusion in aorta tissue were studied.
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spelling pubmed-15673952006-09-19 Monitoring metal concentrations in tissues and single cells using ultramicrosensors. Malinski, T Grunfeld, S Taha, Z Tomboulian, P Environ Health Perspect Research Article Intercellular and extracellular metal concentrations were measured using carbon fiber ultramicrosensors plated with mercury or with polymeric porphyrinic p-type semiconductors. Concentrations of unbound nickel and lead ions were studied within individual BC3H-1 myocytes, and H4-11-C3 rat hepatoma cells. Unbound ions are predominantly solvated inorganic ions not coordinated to biological cellular components. Fabrication of ultramicrosensors appropriate for the cells under investigation is described, including procedures for sharpening and waxing the microsensors in order to control the shape, area, and dimensions of the electroactive surface. Metal ion movement through cell membranes and intracellular ion diffusion in aorta tissue were studied. 1994-09 /pmc/articles/PMC1567395/ /pubmed/7843090 Text en
spellingShingle Research Article
Malinski, T
Grunfeld, S
Taha, Z
Tomboulian, P
Monitoring metal concentrations in tissues and single cells using ultramicrosensors.
title Monitoring metal concentrations in tissues and single cells using ultramicrosensors.
title_full Monitoring metal concentrations in tissues and single cells using ultramicrosensors.
title_fullStr Monitoring metal concentrations in tissues and single cells using ultramicrosensors.
title_full_unstemmed Monitoring metal concentrations in tissues and single cells using ultramicrosensors.
title_short Monitoring metal concentrations in tissues and single cells using ultramicrosensors.
title_sort monitoring metal concentrations in tissues and single cells using ultramicrosensors.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567395/
https://www.ncbi.nlm.nih.gov/pubmed/7843090
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AT grunfelds monitoringmetalconcentrationsintissuesandsinglecellsusingultramicrosensors
AT tahaz monitoringmetalconcentrationsintissuesandsinglecellsusingultramicrosensors
AT tomboulianp monitoringmetalconcentrationsintissuesandsinglecellsusingultramicrosensors

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