Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links.

A rapid, simple, and sensitive 125I-postlabeling technique has been developed to allow detection of DNA-protein cross-links induced by environmental contaminants and carcinogens. This method is based on specific incorporation of 125I into tyrosine residues associated with DNA. Cultured Chinese hamst...

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Detalles Bibliográficos
Autores principales: Zhuang, Z, Costa, M
Formato: Texto
Lenguaje:English
Publicado: 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567398/
https://www.ncbi.nlm.nih.gov/pubmed/7843121
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author Zhuang, Z
Costa, M
author_facet Zhuang, Z
Costa, M
author_sort Zhuang, Z
collection PubMed
description A rapid, simple, and sensitive 125I-postlabeling technique has been developed to allow detection of DNA-protein cross-links induced by environmental contaminants and carcinogens. This method is based on specific incorporation of 125I into tyrosine residues associated with DNA. Cultured Chinese hamster ovary cells were exposed to various crosslinking agents, e.g., UV light, K2CrO4, or NiCl2. DNA was isolated by proteinase K/phenol/chloroform. The residual peptides cross-linked to DNA were radioiodinated with Na125I and chloramine T. After repeated precipitation with ethanol, the radioactivity was determined. The 125I method was compared with a 3[H]-tyrosine prelabeling method and found to be of similar sensitivity.
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spelling pubmed-15673982006-09-19 Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links. Zhuang, Z Costa, M Environ Health Perspect Research Article A rapid, simple, and sensitive 125I-postlabeling technique has been developed to allow detection of DNA-protein cross-links induced by environmental contaminants and carcinogens. This method is based on specific incorporation of 125I into tyrosine residues associated with DNA. Cultured Chinese hamster ovary cells were exposed to various crosslinking agents, e.g., UV light, K2CrO4, or NiCl2. DNA was isolated by proteinase K/phenol/chloroform. The residual peptides cross-linked to DNA were radioiodinated with Na125I and chloramine T. After repeated precipitation with ethanol, the radioactivity was determined. The 125I method was compared with a 3[H]-tyrosine prelabeling method and found to be of similar sensitivity. 1994-09 /pmc/articles/PMC1567398/ /pubmed/7843121 Text en
spellingShingle Research Article
Zhuang, Z
Costa, M
Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links.
title Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links.
title_full Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links.
title_fullStr Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links.
title_full_unstemmed Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links.
title_short Development of an 125I-postlabeling assay as a simple, rapid, and sensitive index of DNA-protein cross-links.
title_sort development of an 125i-postlabeling assay as a simple, rapid, and sensitive index of dna-protein cross-links.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567398/
https://www.ncbi.nlm.nih.gov/pubmed/7843121
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