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Transformation of human osteoblasts to anchorage-independent growth by insoluble nickel particles.

Nickel compounds are well established by epidemiologic studies as human carcinogens. Although the carcinogenicity of nickel compounds has been studied in experimental animals and in a variety of cultured mammalian cells, there are only sporadic reports of nickel-induced transformation of human cells...

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Detalles Bibliográficos
Autores principales: Lin, X, Costa, M
Formato: Texto
Lenguaje:English
Publicado: 1994
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567414/
https://www.ncbi.nlm.nih.gov/pubmed/7843117
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author Lin, X
Costa, M
author_facet Lin, X
Costa, M
author_sort Lin, X
collection PubMed
description Nickel compounds are well established by epidemiologic studies as human carcinogens. Although the carcinogenicity of nickel compounds has been studied in experimental animals and in a variety of cultured mammalian cells, there are only sporadic reports of nickel-induced transformation of human cells. In attempts to study the mechanisms of nickel-induced carcinogenesis in human cells, an immortalized human osteoblastic cell line (HOS) that could not grow in soft agar or form tumors in athymic nude mouse was repeatedly treated with a water-soluble nickel compound (NiCl2) or a less water-soluble nickel compound crystalline (NiS). After three rounds of NiS treatment, there was an increase in anchorage-independent (AI) colony formation. This was not found in untreated or NiCl2-treated cells. Ten AI colonies obtained from NiS-treated cells were isolated. All of these clones showed changes in cell morphology, including the appearance of uniform polygon shape, growth in multilayers, and heavy staining with Giemsa. Most of these clones were retested for their ability to grow in soft agar and showed growth efficiencies of 5 to 50%. It has been shown by other investigators that aggregate growth is well correlated with tumorigenic potential in viral or chemical transformants of HOS cells. Four of seven tested NiS-transformed clones were able to form large aggregates compared to their untransformed counterparts, and continued to proliferate in aggregate form when they were plated on 0.9% agar. Current investigations focus on the molecular and genetic changes induced by nickel compounds in these human cells.
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spelling pubmed-15674142006-09-19 Transformation of human osteoblasts to anchorage-independent growth by insoluble nickel particles. Lin, X Costa, M Environ Health Perspect Research Article Nickel compounds are well established by epidemiologic studies as human carcinogens. Although the carcinogenicity of nickel compounds has been studied in experimental animals and in a variety of cultured mammalian cells, there are only sporadic reports of nickel-induced transformation of human cells. In attempts to study the mechanisms of nickel-induced carcinogenesis in human cells, an immortalized human osteoblastic cell line (HOS) that could not grow in soft agar or form tumors in athymic nude mouse was repeatedly treated with a water-soluble nickel compound (NiCl2) or a less water-soluble nickel compound crystalline (NiS). After three rounds of NiS treatment, there was an increase in anchorage-independent (AI) colony formation. This was not found in untreated or NiCl2-treated cells. Ten AI colonies obtained from NiS-treated cells were isolated. All of these clones showed changes in cell morphology, including the appearance of uniform polygon shape, growth in multilayers, and heavy staining with Giemsa. Most of these clones were retested for their ability to grow in soft agar and showed growth efficiencies of 5 to 50%. It has been shown by other investigators that aggregate growth is well correlated with tumorigenic potential in viral or chemical transformants of HOS cells. Four of seven tested NiS-transformed clones were able to form large aggregates compared to their untransformed counterparts, and continued to proliferate in aggregate form when they were plated on 0.9% agar. Current investigations focus on the molecular and genetic changes induced by nickel compounds in these human cells. 1994-09 /pmc/articles/PMC1567414/ /pubmed/7843117 Text en
spellingShingle Research Article
Lin, X
Costa, M
Transformation of human osteoblasts to anchorage-independent growth by insoluble nickel particles.
title Transformation of human osteoblasts to anchorage-independent growth by insoluble nickel particles.
title_full Transformation of human osteoblasts to anchorage-independent growth by insoluble nickel particles.
title_fullStr Transformation of human osteoblasts to anchorage-independent growth by insoluble nickel particles.
title_full_unstemmed Transformation of human osteoblasts to anchorage-independent growth by insoluble nickel particles.
title_short Transformation of human osteoblasts to anchorage-independent growth by insoluble nickel particles.
title_sort transformation of human osteoblasts to anchorage-independent growth by insoluble nickel particles.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567414/
https://www.ncbi.nlm.nih.gov/pubmed/7843117
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