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Differential cytotoxic effects of arsenic on human and animal cells.

Human fibroblasts (HFW) were 10-fold more susceptible than Chinese hamster ovary (CHO-K1) cells to sodium arsenite. Comparison of cellular antioxidant enzyme activities showed that CHO-K1 cells contained 3- and 8-fold more glutathione-peroxidase and catalase activities, respectively, than HFW cells....

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Detalles Bibliográficos
Autores principales: Lee, T C, Ho, I C
Formato: Texto
Lenguaje:English
Publicado: 1994
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567420/
https://www.ncbi.nlm.nih.gov/pubmed/7843080
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author Lee, T C
Ho, I C
author_facet Lee, T C
Ho, I C
author_sort Lee, T C
collection PubMed
description Human fibroblasts (HFW) were 10-fold more susceptible than Chinese hamster ovary (CHO-K1) cells to sodium arsenite. Comparison of cellular antioxidant enzyme activities showed that CHO-K1 cells contained 3- and 8-fold more glutathione-peroxidase and catalase activities, respectively, than HFW cells. Since vitamin E, methylamine, and benzyl alcohol could prevent, in part, the arsenite-induced killing of HFW cells, we suggest that arsenite can induce oxidative damage in HFW cells. We have also established arsenic-resistant cells, SA7 and CL3R, from CHO cells and from a human lung adenocarcinoma cell line (CL3), respectively. The arsenic resistance of SA7 cells was attributed mainly to elevation of glutathione S-transferase pi levels, and that of CL3R cells was possibly due to an increase in heme oxygenase activity. Since induction of heme oxygenase is a general response to oxidative stress, we suspect that the differential toxicity of arsenic to human and animal cells could be due to arsenic's more efficient induction of oxidative damage in human cells.
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spelling pubmed-15674202006-09-19 Differential cytotoxic effects of arsenic on human and animal cells. Lee, T C Ho, I C Environ Health Perspect Research Article Human fibroblasts (HFW) were 10-fold more susceptible than Chinese hamster ovary (CHO-K1) cells to sodium arsenite. Comparison of cellular antioxidant enzyme activities showed that CHO-K1 cells contained 3- and 8-fold more glutathione-peroxidase and catalase activities, respectively, than HFW cells. Since vitamin E, methylamine, and benzyl alcohol could prevent, in part, the arsenite-induced killing of HFW cells, we suggest that arsenite can induce oxidative damage in HFW cells. We have also established arsenic-resistant cells, SA7 and CL3R, from CHO cells and from a human lung adenocarcinoma cell line (CL3), respectively. The arsenic resistance of SA7 cells was attributed mainly to elevation of glutathione S-transferase pi levels, and that of CL3R cells was possibly due to an increase in heme oxygenase activity. Since induction of heme oxygenase is a general response to oxidative stress, we suspect that the differential toxicity of arsenic to human and animal cells could be due to arsenic's more efficient induction of oxidative damage in human cells. 1994-09 /pmc/articles/PMC1567420/ /pubmed/7843080 Text en
spellingShingle Research Article
Lee, T C
Ho, I C
Differential cytotoxic effects of arsenic on human and animal cells.
title Differential cytotoxic effects of arsenic on human and animal cells.
title_full Differential cytotoxic effects of arsenic on human and animal cells.
title_fullStr Differential cytotoxic effects of arsenic on human and animal cells.
title_full_unstemmed Differential cytotoxic effects of arsenic on human and animal cells.
title_short Differential cytotoxic effects of arsenic on human and animal cells.
title_sort differential cytotoxic effects of arsenic on human and animal cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567420/
https://www.ncbi.nlm.nih.gov/pubmed/7843080
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