Measurement of cytosolic calcium using 19F NMR.

Fluorine-19 nuclear magnetic resonance (NMR) studies of cells and perfused organs loaded with fluorinated ion chelators represent a new approach to determining cytosolic free calcium levels in situ. The molecular basis for this approach and the relative advantages and disadvantages of the NMR techni...

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Detalles Bibliográficos
Autores principales: Murphy, E, Levy, L, Raju, B, Steenbergen, C, Gerig, J T, Singh, P, London, R E
Formato: Texto
Lenguaje:English
Publicado: 1990
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567658/
https://www.ncbi.nlm.nih.gov/pubmed/2351124
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author Murphy, E
Levy, L
Raju, B
Steenbergen, C
Gerig, J T
Singh, P
London, R E
author_facet Murphy, E
Levy, L
Raju, B
Steenbergen, C
Gerig, J T
Singh, P
London, R E
author_sort Murphy, E
collection PubMed
description Fluorine-19 nuclear magnetic resonance (NMR) studies of cells and perfused organs loaded with fluorinated ion chelators represent a new approach to determining cytosolic free calcium levels in situ. The molecular basis for this approach and the relative advantages and disadvantages of the NMR technique are discussed in this paper. Results obtained on perfused normoxic and ischemic rat hearts are presented, indicating that ischemia is associated with an elevation in the level of cytosolic free calcium before the onset of irreversible cell injury. The results are therefore consistent with this elevation playing a causative role in the mediation of myocardial cell injury resulting from ischemia.
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spelling pubmed-15676582006-09-18 Measurement of cytosolic calcium using 19F NMR. Murphy, E Levy, L Raju, B Steenbergen, C Gerig, J T Singh, P London, R E Environ Health Perspect Research Article Fluorine-19 nuclear magnetic resonance (NMR) studies of cells and perfused organs loaded with fluorinated ion chelators represent a new approach to determining cytosolic free calcium levels in situ. The molecular basis for this approach and the relative advantages and disadvantages of the NMR technique are discussed in this paper. Results obtained on perfused normoxic and ischemic rat hearts are presented, indicating that ischemia is associated with an elevation in the level of cytosolic free calcium before the onset of irreversible cell injury. The results are therefore consistent with this elevation playing a causative role in the mediation of myocardial cell injury resulting from ischemia. 1990-03 /pmc/articles/PMC1567658/ /pubmed/2351124 Text en
spellingShingle Research Article
Murphy, E
Levy, L
Raju, B
Steenbergen, C
Gerig, J T
Singh, P
London, R E
Measurement of cytosolic calcium using 19F NMR.
title Measurement of cytosolic calcium using 19F NMR.
title_full Measurement of cytosolic calcium using 19F NMR.
title_fullStr Measurement of cytosolic calcium using 19F NMR.
title_full_unstemmed Measurement of cytosolic calcium using 19F NMR.
title_short Measurement of cytosolic calcium using 19F NMR.
title_sort measurement of cytosolic calcium using 19f nmr.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1567658/
https://www.ncbi.nlm.nih.gov/pubmed/2351124
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