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In vitro studies on the biologic effects of fibers: correlation with in vivo bioassays.

In vitro studies employing organ cultures, primary cell cultures, cell lines, and bacterial systems have been used to assess the toxicity, mutagenicity, and carcinogenic potential of asbestos and nonasbestos fibers. These experiments have been useful in defining mechanisms contributing to the causat...

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Detalles Bibliográficos
Autor principal: Mossman, B T
Formato: Texto
Lenguaje:English
Publicado: 1990
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1568030/
https://www.ncbi.nlm.nih.gov/pubmed/2272329
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author Mossman, B T
author_facet Mossman, B T
author_sort Mossman, B T
collection PubMed
description In vitro studies employing organ cultures, primary cell cultures, cell lines, and bacterial systems have been used to assess the toxicity, mutagenicity, and carcinogenic potential of asbestos and nonasbestos fibers. These experiments have been useful in defining mechanisms contributing to the causation of fiber-associated lung diseases. Long (greater than 8 microns), thin asbestos fibers are more active in vitro than short (less than or equal to 2 microns) fibers or nonfibrous particles, an observation supporting the importance of fiber dimension in disease. Although in vitro bioassays cannot evaluate characteristics such as clearance and/or durability of fibers which may be critical determinants of fiber toxicity in lung, they can be used both to address dosimetry at the cellular level (i.e., number of fibers per cell that elicit a measurable biologic end point) and to evaluate preventive approaches to fiber-induced cell injury. Development of in vitro models employing target cells of the lung, i.e., mesothelial cells, tracheobronchial epithelial cells, and lung fibroblasts, as well as carefully characterized preparations of fibers and particles, will be necessary to evaluate whether in vitro bioassays are amenable to predicting the pathogenic potential of synthetic and naturally occurring fibers comparatively.
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spelling pubmed-15680302006-09-18 In vitro studies on the biologic effects of fibers: correlation with in vivo bioassays. Mossman, B T Environ Health Perspect Research Article In vitro studies employing organ cultures, primary cell cultures, cell lines, and bacterial systems have been used to assess the toxicity, mutagenicity, and carcinogenic potential of asbestos and nonasbestos fibers. These experiments have been useful in defining mechanisms contributing to the causation of fiber-associated lung diseases. Long (greater than 8 microns), thin asbestos fibers are more active in vitro than short (less than or equal to 2 microns) fibers or nonfibrous particles, an observation supporting the importance of fiber dimension in disease. Although in vitro bioassays cannot evaluate characteristics such as clearance and/or durability of fibers which may be critical determinants of fiber toxicity in lung, they can be used both to address dosimetry at the cellular level (i.e., number of fibers per cell that elicit a measurable biologic end point) and to evaluate preventive approaches to fiber-induced cell injury. Development of in vitro models employing target cells of the lung, i.e., mesothelial cells, tracheobronchial epithelial cells, and lung fibroblasts, as well as carefully characterized preparations of fibers and particles, will be necessary to evaluate whether in vitro bioassays are amenable to predicting the pathogenic potential of synthetic and naturally occurring fibers comparatively. 1990-08 /pmc/articles/PMC1568030/ /pubmed/2272329 Text en
spellingShingle Research Article
Mossman, B T
In vitro studies on the biologic effects of fibers: correlation with in vivo bioassays.
title In vitro studies on the biologic effects of fibers: correlation with in vivo bioassays.
title_full In vitro studies on the biologic effects of fibers: correlation with in vivo bioassays.
title_fullStr In vitro studies on the biologic effects of fibers: correlation with in vivo bioassays.
title_full_unstemmed In vitro studies on the biologic effects of fibers: correlation with in vivo bioassays.
title_short In vitro studies on the biologic effects of fibers: correlation with in vivo bioassays.
title_sort in vitro studies on the biologic effects of fibers: correlation with in vivo bioassays.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1568030/
https://www.ncbi.nlm.nih.gov/pubmed/2272329
work_keys_str_mv AT mossmanbt invitrostudiesonthebiologiceffectsoffiberscorrelationwithinvivobioassays