Cargando…
Mechanisms of free radical chemistry and biochemistry of benzene.
omicron-Tyrosine (omicron-Tyr) was used as a specific biomarker for .OH radicals generated in biosystems. Specificity of omicron-Tyr as an .OH biomarker was based on previous studies in systems exposed to ionizing radiations. Fresh muscle tissue incubated with benzene for 1 hr at 38 degrees C exhibi...
Autores principales: | , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
1989
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1568133/ https://www.ncbi.nlm.nih.gov/pubmed/2551667 |
_version_ | 1782129947959099392 |
---|---|
author | Karam, L R Simic, M G |
author_facet | Karam, L R Simic, M G |
author_sort | Karam, L R |
collection | PubMed |
description | omicron-Tyrosine (omicron-Tyr) was used as a specific biomarker for .OH radicals generated in biosystems. Specificity of omicron-Tyr as an .OH biomarker was based on previous studies in systems exposed to ionizing radiations. Fresh muscle tissue incubated with benzene for 1 hr at 38 degrees C exhibits formation of omicron-Tyr as seen in the cases of ethanol- and carbon tetrachloride-exposed systems. Gas chromatography/mass spectrometry selective ion monitoring measurements of omicron-Tyr yields in chicken breast muscle incubated with water or benzene indicate levels of less than 0.1 ppm and 3.0 +/- 0.5 ppm of omicron-Tyr, respectively. Formation of .OH is presumed to originate via a Haber-Weiss reaction of H2O2 with Fe(II) preceded by the formation of .O2- and H2O2 from distorted mitochondria. |
format | Text |
id | pubmed-1568133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1989 |
record_format | MEDLINE/PubMed |
spelling | pubmed-15681332006-09-18 Mechanisms of free radical chemistry and biochemistry of benzene. Karam, L R Simic, M G Environ Health Perspect Research Article omicron-Tyrosine (omicron-Tyr) was used as a specific biomarker for .OH radicals generated in biosystems. Specificity of omicron-Tyr as an .OH biomarker was based on previous studies in systems exposed to ionizing radiations. Fresh muscle tissue incubated with benzene for 1 hr at 38 degrees C exhibits formation of omicron-Tyr as seen in the cases of ethanol- and carbon tetrachloride-exposed systems. Gas chromatography/mass spectrometry selective ion monitoring measurements of omicron-Tyr yields in chicken breast muscle incubated with water or benzene indicate levels of less than 0.1 ppm and 3.0 +/- 0.5 ppm of omicron-Tyr, respectively. Formation of .OH is presumed to originate via a Haber-Weiss reaction of H2O2 with Fe(II) preceded by the formation of .O2- and H2O2 from distorted mitochondria. 1989-07 /pmc/articles/PMC1568133/ /pubmed/2551667 Text en |
spellingShingle | Research Article Karam, L R Simic, M G Mechanisms of free radical chemistry and biochemistry of benzene. |
title | Mechanisms of free radical chemistry and biochemistry of benzene. |
title_full | Mechanisms of free radical chemistry and biochemistry of benzene. |
title_fullStr | Mechanisms of free radical chemistry and biochemistry of benzene. |
title_full_unstemmed | Mechanisms of free radical chemistry and biochemistry of benzene. |
title_short | Mechanisms of free radical chemistry and biochemistry of benzene. |
title_sort | mechanisms of free radical chemistry and biochemistry of benzene. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1568133/ https://www.ncbi.nlm.nih.gov/pubmed/2551667 |
work_keys_str_mv | AT karamlr mechanismsoffreeradicalchemistryandbiochemistryofbenzene AT simicmg mechanismsoffreeradicalchemistryandbiochemistryofbenzene |