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Activation of bone marrow phagocytes following benzene treatment of mice.

Techniques in flow cytometry/cell sorting were used to characterize the effects of benzene and its metabolites on subpopulations of bone marrow cells. Treatment of male Balb/c mice with benzene (880 mg/kg) or a combination of its metabolites, hydroquinone and phenol (50 mg/kg), resulted in a 30 to 4...

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Detalles Bibliográficos
Autores principales: Laskin, D L, MacEachern, L, Snyder, R
Formato: Texto
Lenguaje:English
Publicado: 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1568140/
https://www.ncbi.nlm.nih.gov/pubmed/2676504
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author Laskin, D L
MacEachern, L
Snyder, R
author_facet Laskin, D L
MacEachern, L
Snyder, R
author_sort Laskin, D L
collection PubMed
description Techniques in flow cytometry/cell sorting were used to characterize the effects of benzene and its metabolites on subpopulations of bone marrow cells. Treatment of male Balb/c mice with benzene (880 mg/kg) or a combination of its metabolites, hydroquinone and phenol (50 mg/kg), resulted in a 30 to 40% decrease in bone marrow cellularity. Flow cytometric analysis revealed two subpopulations of bone marrow cells that could be distinguished by their size and density or granularity. The larger, more dense subpopulation was found to consist predominantly of macrophages and granulocytes as determined by monoclonal antibody binding and by cell sorting. Benzene treatment had no selective cytotoxic effects on subpopulations of bone marrow cells. To determine if benzene treatment activated bone marrow phagocytes, we quantified production of hydrogen peroxide by these cells using the fluorescent indicator dye, 2',7'-dichlorofluorescein diacetate. We found that macrophages and granulocytes from bone marrow of treated mice produced 50% more hydrogen peroxide in response to the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate than did cells from control animals. It is hypothesized that phagocyte activation and production of cytotoxic reactive oxygen intermediates may contribute to hematotoxicity induced by benzene.
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spelling pubmed-15681402006-09-18 Activation of bone marrow phagocytes following benzene treatment of mice. Laskin, D L MacEachern, L Snyder, R Environ Health Perspect Research Article Techniques in flow cytometry/cell sorting were used to characterize the effects of benzene and its metabolites on subpopulations of bone marrow cells. Treatment of male Balb/c mice with benzene (880 mg/kg) or a combination of its metabolites, hydroquinone and phenol (50 mg/kg), resulted in a 30 to 40% decrease in bone marrow cellularity. Flow cytometric analysis revealed two subpopulations of bone marrow cells that could be distinguished by their size and density or granularity. The larger, more dense subpopulation was found to consist predominantly of macrophages and granulocytes as determined by monoclonal antibody binding and by cell sorting. Benzene treatment had no selective cytotoxic effects on subpopulations of bone marrow cells. To determine if benzene treatment activated bone marrow phagocytes, we quantified production of hydrogen peroxide by these cells using the fluorescent indicator dye, 2',7'-dichlorofluorescein diacetate. We found that macrophages and granulocytes from bone marrow of treated mice produced 50% more hydrogen peroxide in response to the phorbol ester, 12-O-tetradecanoyl-phorbol-13-acetate than did cells from control animals. It is hypothesized that phagocyte activation and production of cytotoxic reactive oxygen intermediates may contribute to hematotoxicity induced by benzene. 1989-07 /pmc/articles/PMC1568140/ /pubmed/2676504 Text en
spellingShingle Research Article
Laskin, D L
MacEachern, L
Snyder, R
Activation of bone marrow phagocytes following benzene treatment of mice.
title Activation of bone marrow phagocytes following benzene treatment of mice.
title_full Activation of bone marrow phagocytes following benzene treatment of mice.
title_fullStr Activation of bone marrow phagocytes following benzene treatment of mice.
title_full_unstemmed Activation of bone marrow phagocytes following benzene treatment of mice.
title_short Activation of bone marrow phagocytes following benzene treatment of mice.
title_sort activation of bone marrow phagocytes following benzene treatment of mice.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1568140/
https://www.ncbi.nlm.nih.gov/pubmed/2676504
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