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Isolation of Clara cells from the mouse lung.
A method is described for isolating Clara cells from the mouse lung that does not require the technique of elutriation. Mouse lungs totally perfused of blood are instilled with crystalline trypsin (0.25%) and incubated for the optimum time of 15 min. The lung tissue is chopped, mechanically agitated...
Autores principales: | Oreffo, V I, Morgan, A, Richards, R J |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
1990
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1568317/ https://www.ncbi.nlm.nih.gov/pubmed/2200669 |
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