Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.

Methods are being developed for derivatizing trace amounts of DNA adducts for ultimate determination by gas chromatographic techniques. High-pressure liquid chromatography is used to optimize appropriate derivatization reactions for the determination of cytosine. A single vial reaction scheme involv...

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Detalles Bibliográficos
Autores principales: Fisher, D H, Adams, J, Giese, R W
Formato: Texto
Lenguaje:English
Publicado: 1985
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1568697/
https://www.ncbi.nlm.nih.gov/pubmed/4085449
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author Fisher, D H
Adams, J
Giese, R W
author_facet Fisher, D H
Adams, J
Giese, R W
author_sort Fisher, D H
collection PubMed
description Methods are being developed for derivatizing trace amounts of DNA adducts for ultimate determination by gas chromatographic techniques. High-pressure liquid chromatography is used to optimize appropriate derivatization reactions for the determination of cytosine. A single vial reaction scheme involves acylation with electrophoric pentafluorobenzoylchloride followed by alkylation with dimethyl sulfate. Currently, the overall yield for this reaction starting with 50 nmole of cytosine is 59 +/- 4.6%.
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spelling pubmed-15686972006-09-18 Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate. Fisher, D H Adams, J Giese, R W Environ Health Perspect Research Article Methods are being developed for derivatizing trace amounts of DNA adducts for ultimate determination by gas chromatographic techniques. High-pressure liquid chromatography is used to optimize appropriate derivatization reactions for the determination of cytosine. A single vial reaction scheme involves acylation with electrophoric pentafluorobenzoylchloride followed by alkylation with dimethyl sulfate. Currently, the overall yield for this reaction starting with 50 nmole of cytosine is 59 +/- 4.6%. 1985-10 /pmc/articles/PMC1568697/ /pubmed/4085449 Text en
spellingShingle Research Article
Fisher, D H
Adams, J
Giese, R W
Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.
title Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.
title_full Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.
title_fullStr Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.
title_full_unstemmed Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.
title_short Trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.
title_sort trace derivatization of cytosine with pentafluorobenzoyl chloride and dimethyl sulfate.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1568697/
https://www.ncbi.nlm.nih.gov/pubmed/4085449
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AT adamsj tracederivatizationofcytosinewithpentafluorobenzoylchlorideanddimethylsulfate
AT gieserw tracederivatizationofcytosinewithpentafluorobenzoylchlorideanddimethylsulfate

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