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Sandwich hybridisation assay for quantitative detection of yeast RNAs in crude cell lysates
BACKGROUND: A rapid microtiter plate based sandwich hybridization assay was developed for detection and quantification of single RNA species using magnetic beads. Following solution hybridization target RNA molecules were collected by biotin-streptavidin affinity binding and detected by fluorescence...
| Autores principales: | , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2003
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC156893/ https://www.ncbi.nlm.nih.gov/pubmed/12780940 http://dx.doi.org/10.1186/1475-2859-2-4 |