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An adherent cell perifusion technique to study the overall and sequential response of rat alveolar macrophages to toxic substances.
Essentially pure (97%) alveolar macrophages were isolated by bronchoalveolar lavage of rats with warm (37 degrees C) PBS solution. These cells were allowed to adhere to the inside walls of open-ended glass cylinders which were closed off at each end by three-way stopcocks. The adhering cells were pe...
Autores principales: | Forget, G, Lacroix, M J, Cadieux, A, Calvert, R, Grose, J H, Sirois, P |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
1983
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1569300/ https://www.ncbi.nlm.nih.gov/pubmed/6641651 |
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