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Development of an in vitro model to study clot lysis activity of thrombolytic drugs
BACKGROUND: Thrombolytic drugs are widely used for the management of cerebral venous sinus thrombosis patients. Several in vitro models have been developed to study clot lytic activity of thrombolytic drugs, but all of these have certain limitations. There is need of an appropriate model to check th...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1570448/ https://www.ncbi.nlm.nih.gov/pubmed/16968529 http://dx.doi.org/10.1186/1477-9560-4-14 |
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author | Prasad, Sweta Kashyap, Rajpal S Deopujari, Jayant Y Purohit, Hemant J Taori, Girdhar M Daginawala, Hatim F |
author_facet | Prasad, Sweta Kashyap, Rajpal S Deopujari, Jayant Y Purohit, Hemant J Taori, Girdhar M Daginawala, Hatim F |
author_sort | Prasad, Sweta |
collection | PubMed |
description | BACKGROUND: Thrombolytic drugs are widely used for the management of cerebral venous sinus thrombosis patients. Several in vitro models have been developed to study clot lytic activity of thrombolytic drugs, but all of these have certain limitations. There is need of an appropriate model to check the clot lytic efficacy of thrombolytic drugs. In the present study, an attempt has been made to design and develop a new model system to study clot lysis in a simplified and easy way using a thrombolytic drug, streptokinase. METHODS: Whole blood from healthy individuals (n = 20) was allowed to form clots in a pre-weighed sterile microcentrifuge tubes; serum was removed and clot was weighed. After lysis by streptokinase fluid was removed and remnants of clot were again weighed along with the tube. Percentage of Clot lysis was calculated on the basis of the weight difference of microcentrifuge tubes obtained before and after clot lysis. RESULTS: There was a significant percentage of clot lysis observed when streptokinase was used. On the other hand with water (negative control), minimal (2.5%) clot lysis was observed. There was a significant difference between clot lysis done by streptokinase and water. CONCLUSION: Our study could be a rapid and effective methodology to study clot-lytic effect of newly developed drugs as well as known drugs. |
format | Text |
id | pubmed-1570448 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-15704482006-09-21 Development of an in vitro model to study clot lysis activity of thrombolytic drugs Prasad, Sweta Kashyap, Rajpal S Deopujari, Jayant Y Purohit, Hemant J Taori, Girdhar M Daginawala, Hatim F Thromb J Original Basic Research BACKGROUND: Thrombolytic drugs are widely used for the management of cerebral venous sinus thrombosis patients. Several in vitro models have been developed to study clot lytic activity of thrombolytic drugs, but all of these have certain limitations. There is need of an appropriate model to check the clot lytic efficacy of thrombolytic drugs. In the present study, an attempt has been made to design and develop a new model system to study clot lysis in a simplified and easy way using a thrombolytic drug, streptokinase. METHODS: Whole blood from healthy individuals (n = 20) was allowed to form clots in a pre-weighed sterile microcentrifuge tubes; serum was removed and clot was weighed. After lysis by streptokinase fluid was removed and remnants of clot were again weighed along with the tube. Percentage of Clot lysis was calculated on the basis of the weight difference of microcentrifuge tubes obtained before and after clot lysis. RESULTS: There was a significant percentage of clot lysis observed when streptokinase was used. On the other hand with water (negative control), minimal (2.5%) clot lysis was observed. There was a significant difference between clot lysis done by streptokinase and water. CONCLUSION: Our study could be a rapid and effective methodology to study clot-lytic effect of newly developed drugs as well as known drugs. BioMed Central 2006-09-12 /pmc/articles/PMC1570448/ /pubmed/16968529 http://dx.doi.org/10.1186/1477-9560-4-14 Text en Copyright © 2006 Prasad et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Basic Research Prasad, Sweta Kashyap, Rajpal S Deopujari, Jayant Y Purohit, Hemant J Taori, Girdhar M Daginawala, Hatim F Development of an in vitro model to study clot lysis activity of thrombolytic drugs |
title | Development of an in vitro model to study clot lysis activity of thrombolytic drugs |
title_full | Development of an in vitro model to study clot lysis activity of thrombolytic drugs |
title_fullStr | Development of an in vitro model to study clot lysis activity of thrombolytic drugs |
title_full_unstemmed | Development of an in vitro model to study clot lysis activity of thrombolytic drugs |
title_short | Development of an in vitro model to study clot lysis activity of thrombolytic drugs |
title_sort | development of an in vitro model to study clot lysis activity of thrombolytic drugs |
topic | Original Basic Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1570448/ https://www.ncbi.nlm.nih.gov/pubmed/16968529 http://dx.doi.org/10.1186/1477-9560-4-14 |
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