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Leishmania isoenzyme polymorphisms in Ecuador: Relationships with geographic distribution and clinical presentation
BACKGROUND: Determinants of the clinical presentation of the leishmaniases are poorly understood but Leishmania species and strain differences are important. To examine the relationship between clinical presentation, species and isoenzyme polymorphisms, 56 Leishmania isolates from distinct presentat...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1578576/ https://www.ncbi.nlm.nih.gov/pubmed/16968553 http://dx.doi.org/10.1186/1471-2334-6-139 |
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author | Calvopina, Manuel Armijos, Rodrigo X Marco, Jorge D Uezato, Hiroshi Kato, Hirotomo Gomez, Eduardo A Korenaga, Masataka Barroso, Paola A Mimori, Tatsuyuki Cooper, Philip J Nonaka, Shigeo Hashiguchi, Yoshihisa |
author_facet | Calvopina, Manuel Armijos, Rodrigo X Marco, Jorge D Uezato, Hiroshi Kato, Hirotomo Gomez, Eduardo A Korenaga, Masataka Barroso, Paola A Mimori, Tatsuyuki Cooper, Philip J Nonaka, Shigeo Hashiguchi, Yoshihisa |
author_sort | Calvopina, Manuel |
collection | PubMed |
description | BACKGROUND: Determinants of the clinical presentation of the leishmaniases are poorly understood but Leishmania species and strain differences are important. To examine the relationship between clinical presentation, species and isoenzyme polymorphisms, 56 Leishmania isolates from distinct presentations of American tegumentary leishmaniasis (ATL) from Ecuador were analyzed. METHODS: Isolates were characterized by multilocus enzyme electrophoresis for polymorphisms of 11 isoenzymes. Patients were infected in four different ecologic regions: highland and lowland jungle of the Pacific coast, Amazonian lowlands and Andean highlands. RESULTS: Six Leishmania species constituting 21 zymodemes were identified: L. (Viannia) panamensis (21 isolates, 7 zymodemes), L. (V.) guyanensis (7 isolates, 4 zymodemes), L. (V.) braziliensis (5 isolates, 3 zymodemes), L. (Leishmania) mexicana (11 isolates, 4 zymodemes), L. (L.) amazonensis (10 isolates, 2 zymodemes) and L. (L.) major (2 isolates, 1 zymodeme). L. panamensis was the species most frequently identified in the Pacific region and was associated with several clinical variants of cutaneous disease (CL); eight cases of leishmaniasis recidiva cutis (LRC) found in the Pacific highlands were associated with 3 zymodemes of this species. Mucocutaneous leishmaniasis found only in the Amazonian focus was associated with 3 zymodemes of L. braziliensis. The papular variant of CL, Uta, found in the Andean highlands was related predominantly with a single zymodeme of L. mexicana. CONCLUSION: Our data show a high degree of phenotypic variation within species, and some evidence for associations between specific variants of ATL (i.e. Uta and LRC) and specific Leishmania zymodemes. This study further defines the geographic distribution of Leishmania species and clinical variants of ATL in Ecuador. |
format | Text |
id | pubmed-1578576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-15785762006-09-27 Leishmania isoenzyme polymorphisms in Ecuador: Relationships with geographic distribution and clinical presentation Calvopina, Manuel Armijos, Rodrigo X Marco, Jorge D Uezato, Hiroshi Kato, Hirotomo Gomez, Eduardo A Korenaga, Masataka Barroso, Paola A Mimori, Tatsuyuki Cooper, Philip J Nonaka, Shigeo Hashiguchi, Yoshihisa BMC Infect Dis Research Article BACKGROUND: Determinants of the clinical presentation of the leishmaniases are poorly understood but Leishmania species and strain differences are important. To examine the relationship between clinical presentation, species and isoenzyme polymorphisms, 56 Leishmania isolates from distinct presentations of American tegumentary leishmaniasis (ATL) from Ecuador were analyzed. METHODS: Isolates were characterized by multilocus enzyme electrophoresis for polymorphisms of 11 isoenzymes. Patients were infected in four different ecologic regions: highland and lowland jungle of the Pacific coast, Amazonian lowlands and Andean highlands. RESULTS: Six Leishmania species constituting 21 zymodemes were identified: L. (Viannia) panamensis (21 isolates, 7 zymodemes), L. (V.) guyanensis (7 isolates, 4 zymodemes), L. (V.) braziliensis (5 isolates, 3 zymodemes), L. (Leishmania) mexicana (11 isolates, 4 zymodemes), L. (L.) amazonensis (10 isolates, 2 zymodemes) and L. (L.) major (2 isolates, 1 zymodeme). L. panamensis was the species most frequently identified in the Pacific region and was associated with several clinical variants of cutaneous disease (CL); eight cases of leishmaniasis recidiva cutis (LRC) found in the Pacific highlands were associated with 3 zymodemes of this species. Mucocutaneous leishmaniasis found only in the Amazonian focus was associated with 3 zymodemes of L. braziliensis. The papular variant of CL, Uta, found in the Andean highlands was related predominantly with a single zymodeme of L. mexicana. CONCLUSION: Our data show a high degree of phenotypic variation within species, and some evidence for associations between specific variants of ATL (i.e. Uta and LRC) and specific Leishmania zymodemes. This study further defines the geographic distribution of Leishmania species and clinical variants of ATL in Ecuador. BioMed Central 2006-09-13 /pmc/articles/PMC1578576/ /pubmed/16968553 http://dx.doi.org/10.1186/1471-2334-6-139 Text en Copyright © 2006 Calvopina et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Calvopina, Manuel Armijos, Rodrigo X Marco, Jorge D Uezato, Hiroshi Kato, Hirotomo Gomez, Eduardo A Korenaga, Masataka Barroso, Paola A Mimori, Tatsuyuki Cooper, Philip J Nonaka, Shigeo Hashiguchi, Yoshihisa Leishmania isoenzyme polymorphisms in Ecuador: Relationships with geographic distribution and clinical presentation |
title | Leishmania isoenzyme polymorphisms in Ecuador: Relationships with geographic distribution and clinical presentation |
title_full | Leishmania isoenzyme polymorphisms in Ecuador: Relationships with geographic distribution and clinical presentation |
title_fullStr | Leishmania isoenzyme polymorphisms in Ecuador: Relationships with geographic distribution and clinical presentation |
title_full_unstemmed | Leishmania isoenzyme polymorphisms in Ecuador: Relationships with geographic distribution and clinical presentation |
title_short | Leishmania isoenzyme polymorphisms in Ecuador: Relationships with geographic distribution and clinical presentation |
title_sort | leishmania isoenzyme polymorphisms in ecuador: relationships with geographic distribution and clinical presentation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1578576/ https://www.ncbi.nlm.nih.gov/pubmed/16968553 http://dx.doi.org/10.1186/1471-2334-6-139 |
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