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Observation of the low frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy

BACKGROUND: Recently, a technique which departs radically from conventional approaches has been proposed. This novel technique utilizes biological objects such as viruses as nano-templates for the fabrication of nanostructure elements. For example, rod-shaped viruses such as the M13 phage and tobacc...

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Autores principales: Tsen, KT, Dykeman, Eric C, Sankey, Otto F, Lin, Nien-Tsung, Tsen, Shaw-Wei D, Kiang, Juliann G
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1592082/
https://www.ncbi.nlm.nih.gov/pubmed/16995944
http://dx.doi.org/10.1186/1743-422X-3-79
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author Tsen, KT
Dykeman, Eric C
Sankey, Otto F
Lin, Nien-Tsung
Tsen, Shaw-Wei D
Kiang, Juliann G
author_facet Tsen, KT
Dykeman, Eric C
Sankey, Otto F
Lin, Nien-Tsung
Tsen, Shaw-Wei D
Kiang, Juliann G
author_sort Tsen, KT
collection PubMed
description BACKGROUND: Recently, a technique which departs radically from conventional approaches has been proposed. This novel technique utilizes biological objects such as viruses as nano-templates for the fabrication of nanostructure elements. For example, rod-shaped viruses such as the M13 phage and tobacco mosaic virus have been successfully used as biological templates for the synthesis of semiconductor and metallic nanowires. RESULTS AND DISCUSSION: Low wave number (≤ 20 cm(-1)) acoustic vibrations of the M13 phage have been studied using Raman spectroscopy. The experimental results are compared with theoretical calculations based on an elastic continuum model and appropriate Raman selection rules derived from a bond polarizability model. The observed Raman mode has been shown to belong to one of the Raman-active axial torsion modes of the M13 phage protein coat. CONCLUSION: It is expected that the detection and characterization of this low frequency vibrational mode can be used for applications in nanotechnology such as for monitoring the process of virus functionalization and self-assembly. For example, the differences in Raman spectra can be used to monitor the coating of virus with some other materials and nano-assembly process, such as attaching a carbon nanotube or quantum dots.
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spelling pubmed-15920822006-10-05 Observation of the low frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy Tsen, KT Dykeman, Eric C Sankey, Otto F Lin, Nien-Tsung Tsen, Shaw-Wei D Kiang, Juliann G Virol J Research BACKGROUND: Recently, a technique which departs radically from conventional approaches has been proposed. This novel technique utilizes biological objects such as viruses as nano-templates for the fabrication of nanostructure elements. For example, rod-shaped viruses such as the M13 phage and tobacco mosaic virus have been successfully used as biological templates for the synthesis of semiconductor and metallic nanowires. RESULTS AND DISCUSSION: Low wave number (≤ 20 cm(-1)) acoustic vibrations of the M13 phage have been studied using Raman spectroscopy. The experimental results are compared with theoretical calculations based on an elastic continuum model and appropriate Raman selection rules derived from a bond polarizability model. The observed Raman mode has been shown to belong to one of the Raman-active axial torsion modes of the M13 phage protein coat. CONCLUSION: It is expected that the detection and characterization of this low frequency vibrational mode can be used for applications in nanotechnology such as for monitoring the process of virus functionalization and self-assembly. For example, the differences in Raman spectra can be used to monitor the coating of virus with some other materials and nano-assembly process, such as attaching a carbon nanotube or quantum dots. BioMed Central 2006-09-22 /pmc/articles/PMC1592082/ /pubmed/16995944 http://dx.doi.org/10.1186/1743-422X-3-79 Text en Copyright © 2006 Tsen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Tsen, KT
Dykeman, Eric C
Sankey, Otto F
Lin, Nien-Tsung
Tsen, Shaw-Wei D
Kiang, Juliann G
Observation of the low frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy
title Observation of the low frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy
title_full Observation of the low frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy
title_fullStr Observation of the low frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy
title_full_unstemmed Observation of the low frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy
title_short Observation of the low frequency vibrational modes of bacteriophage M13 in water by Raman spectroscopy
title_sort observation of the low frequency vibrational modes of bacteriophage m13 in water by raman spectroscopy
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1592082/
https://www.ncbi.nlm.nih.gov/pubmed/16995944
http://dx.doi.org/10.1186/1743-422X-3-79
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