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S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt

BACKGROUND: Infectious bronchitis is highly contagious and constitutes one of the most common and difficult poultry diseases to control. IBV is endemic in probably all countries that raise chickens. It exists as dozens of serotypes/genotypes. Only a few amino acid differences in the S1 protein of va...

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Autores principales: Abdel-Moneim, Ahmed S, El-Kady, Magdy F, Ladman, Brian S, Gelb, Jack
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1592083/
https://www.ncbi.nlm.nih.gov/pubmed/16987422
http://dx.doi.org/10.1186/1743-422X-3-78
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author Abdel-Moneim, Ahmed S
El-Kady, Magdy F
Ladman, Brian S
Gelb, Jack
author_facet Abdel-Moneim, Ahmed S
El-Kady, Magdy F
Ladman, Brian S
Gelb, Jack
author_sort Abdel-Moneim, Ahmed S
collection PubMed
description BACKGROUND: Infectious bronchitis is highly contagious and constitutes one of the most common and difficult poultry diseases to control. IBV is endemic in probably all countries that raise chickens. It exists as dozens of serotypes/genotypes. Only a few amino acid differences in the S1 protein of vaccine and challenge strains of IBV may result in poor protection. Tropism of IBV includes the respiratory tract tissues, proventriculus and caecal tonsils of the alimentary tract, the oviduct and the kidney. RESULTS: Infectious bronchitis virus (IBV) strain closely related to Massachusetts (Mass) serotype was isolated from broiler chickens suffering from severe renal and respiratory distresses. The isolate was serologically identified by Dot-ELISA and further characterized by RT-PCR then genotyped using S1 gene sequence analysis. Alignment of the S1 sequence of the isolate with 16 IBV strains revealed high homology to isolates related to Mass serotype. Inoculation with the strain reproduced the disease in experimental 1-day-old chickens and resulted in 20% mortality, severe renal and moderate respiratory distresses. Marked histopathological changes in both kidney and trachea were observed in experimentally infected chickens. A protection study using the H120 live attenuated vaccine showed low protection rate in spite of high S1 sequence homology (97%). Protection based criteria were: virus re-isolation attempts from trachea, tracheal and renal histopathology as well as IBV antigens detection by immunofluorescent antibody technique in kidney sections. CONCLUSION: Periodical evaluation of cross-protective capabilities of IBV vaccine(s) versus recently recovered field isolates should be performed to ensure optimum control of IBV.
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spelling pubmed-15920832006-10-05 S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt Abdel-Moneim, Ahmed S El-Kady, Magdy F Ladman, Brian S Gelb, Jack Virol J Research BACKGROUND: Infectious bronchitis is highly contagious and constitutes one of the most common and difficult poultry diseases to control. IBV is endemic in probably all countries that raise chickens. It exists as dozens of serotypes/genotypes. Only a few amino acid differences in the S1 protein of vaccine and challenge strains of IBV may result in poor protection. Tropism of IBV includes the respiratory tract tissues, proventriculus and caecal tonsils of the alimentary tract, the oviduct and the kidney. RESULTS: Infectious bronchitis virus (IBV) strain closely related to Massachusetts (Mass) serotype was isolated from broiler chickens suffering from severe renal and respiratory distresses. The isolate was serologically identified by Dot-ELISA and further characterized by RT-PCR then genotyped using S1 gene sequence analysis. Alignment of the S1 sequence of the isolate with 16 IBV strains revealed high homology to isolates related to Mass serotype. Inoculation with the strain reproduced the disease in experimental 1-day-old chickens and resulted in 20% mortality, severe renal and moderate respiratory distresses. Marked histopathological changes in both kidney and trachea were observed in experimentally infected chickens. A protection study using the H120 live attenuated vaccine showed low protection rate in spite of high S1 sequence homology (97%). Protection based criteria were: virus re-isolation attempts from trachea, tracheal and renal histopathology as well as IBV antigens detection by immunofluorescent antibody technique in kidney sections. CONCLUSION: Periodical evaluation of cross-protective capabilities of IBV vaccine(s) versus recently recovered field isolates should be performed to ensure optimum control of IBV. BioMed Central 2006-09-20 /pmc/articles/PMC1592083/ /pubmed/16987422 http://dx.doi.org/10.1186/1743-422X-3-78 Text en Copyright © 2006 Abdel-Moneim et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Abdel-Moneim, Ahmed S
El-Kady, Magdy F
Ladman, Brian S
Gelb, Jack
S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt
title S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt
title_full S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt
title_fullStr S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt
title_full_unstemmed S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt
title_short S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt
title_sort s1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in egypt
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1592083/
https://www.ncbi.nlm.nih.gov/pubmed/16987422
http://dx.doi.org/10.1186/1743-422X-3-78
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