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Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system 'FocusField2-6GallupqPCRSet-upTool-001' to attain consistently high fidelity qPCR reactions
The purpose of this manuscript is to discuss fluorogenic real-time quantitative polymerase chain reaction (qPCR) inhibition and to introduce/define a novel Microsoft Excel-based file system which provides a way to detect and avoid inhibition, and enables investigators to consistently design dynamica...
Autores principales: | Gallup, Jack M., Ackermann, Mark R. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Biological Procedures Online
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1592462/ https://www.ncbi.nlm.nih.gov/pubmed/17033699 http://dx.doi.org/10.1251/bpo122 |
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