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Eukaryotic Initiation Factor 4E (eIF4E) and angiogenesis: prognostic markers for breast cancer
BACKGROUND: The overexpression of eukaryotic translation initiation factor 4E (eIF4E), a key regulator of protein synthesis, is involved in the malignant progression of human breast cancer. This study investigates the relationship between eIF4E and angiogenesis, as well as their prognostic impact in...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1599748/ https://www.ncbi.nlm.nih.gov/pubmed/17010208 http://dx.doi.org/10.1186/1471-2407-6-231 |
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author | Zhou, Sheng Wang, Guo-Ping Liu, Cong Zhou, Muxiang |
author_facet | Zhou, Sheng Wang, Guo-Ping Liu, Cong Zhou, Muxiang |
author_sort | Zhou, Sheng |
collection | PubMed |
description | BACKGROUND: The overexpression of eukaryotic translation initiation factor 4E (eIF4E), a key regulator of protein synthesis, is involved in the malignant progression of human breast cancer. This study investigates the relationship between eIF4E and angiogenesis, as well as their prognostic impact in patients with human breast cancer. METHODS: Immunohistochemical staining was used to determine protein expression of eIF4E, vascular endothelial growth factor (VEGF), interleukin-8 (IL-8), and CD105 in a set of 122 formalin-fixed, paraffin-embedded primary breast cancer tissues. Expression of eIF4E in positive cells was characterized by cytoplasmic staining. Evaluation of VEGF and IL-8 in the same tissue established the angiogenic profiles, while CD105 was used as an indicator of microvessel density (MVD). RESULTS: A significant relationship was found between the level of eIF4E expression and histological grade (P = 0.016). VEGF, IL-8, and MVD were closely related to tumor grade (P = 0.003, P = 0.022, and P < 0.001, respectively) and clinical stage (P = 0.007, P = 0.048, and P < 0.001, respectively). Expression of eIF4E was also significantly correlated with VEGF (P = 0.007), IL-8 (P = 0.007), and MVD (P = 0.006). Patients overexpressing eIF4E had significantly worse overall (P = 0.01) and disease-free survival (P = 0.006). When eIF4E, histological grade, tumor stage, ER, PR, Her-2 status and the levels of VEGF, IL-8, MVD were included in a multivariate Cox regression analysis, eIF4E emerged as an independent prognostic factor for breast cancer (P = 0.001), along with stage (P = 0.005), node status (P = 0.046), and MVD (P = 0.004). CONCLUSION: These results suggest that higher eIF4E expression correlates with both angiogenesis and vascular invasion of cancer cells, and could therefore serve as a useful histological predictor for less favorable outcome in breast cancer patients, as well as represent a potential therapeutic target. |
format | Text |
id | pubmed-1599748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-15997482006-10-12 Eukaryotic Initiation Factor 4E (eIF4E) and angiogenesis: prognostic markers for breast cancer Zhou, Sheng Wang, Guo-Ping Liu, Cong Zhou, Muxiang BMC Cancer Research Article BACKGROUND: The overexpression of eukaryotic translation initiation factor 4E (eIF4E), a key regulator of protein synthesis, is involved in the malignant progression of human breast cancer. This study investigates the relationship between eIF4E and angiogenesis, as well as their prognostic impact in patients with human breast cancer. METHODS: Immunohistochemical staining was used to determine protein expression of eIF4E, vascular endothelial growth factor (VEGF), interleukin-8 (IL-8), and CD105 in a set of 122 formalin-fixed, paraffin-embedded primary breast cancer tissues. Expression of eIF4E in positive cells was characterized by cytoplasmic staining. Evaluation of VEGF and IL-8 in the same tissue established the angiogenic profiles, while CD105 was used as an indicator of microvessel density (MVD). RESULTS: A significant relationship was found between the level of eIF4E expression and histological grade (P = 0.016). VEGF, IL-8, and MVD were closely related to tumor grade (P = 0.003, P = 0.022, and P < 0.001, respectively) and clinical stage (P = 0.007, P = 0.048, and P < 0.001, respectively). Expression of eIF4E was also significantly correlated with VEGF (P = 0.007), IL-8 (P = 0.007), and MVD (P = 0.006). Patients overexpressing eIF4E had significantly worse overall (P = 0.01) and disease-free survival (P = 0.006). When eIF4E, histological grade, tumor stage, ER, PR, Her-2 status and the levels of VEGF, IL-8, MVD were included in a multivariate Cox regression analysis, eIF4E emerged as an independent prognostic factor for breast cancer (P = 0.001), along with stage (P = 0.005), node status (P = 0.046), and MVD (P = 0.004). CONCLUSION: These results suggest that higher eIF4E expression correlates with both angiogenesis and vascular invasion of cancer cells, and could therefore serve as a useful histological predictor for less favorable outcome in breast cancer patients, as well as represent a potential therapeutic target. BioMed Central 2006-09-30 /pmc/articles/PMC1599748/ /pubmed/17010208 http://dx.doi.org/10.1186/1471-2407-6-231 Text en Copyright © 2006 Zhou et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhou, Sheng Wang, Guo-Ping Liu, Cong Zhou, Muxiang Eukaryotic Initiation Factor 4E (eIF4E) and angiogenesis: prognostic markers for breast cancer |
title | Eukaryotic Initiation Factor 4E (eIF4E) and angiogenesis: prognostic markers for breast cancer |
title_full | Eukaryotic Initiation Factor 4E (eIF4E) and angiogenesis: prognostic markers for breast cancer |
title_fullStr | Eukaryotic Initiation Factor 4E (eIF4E) and angiogenesis: prognostic markers for breast cancer |
title_full_unstemmed | Eukaryotic Initiation Factor 4E (eIF4E) and angiogenesis: prognostic markers for breast cancer |
title_short | Eukaryotic Initiation Factor 4E (eIF4E) and angiogenesis: prognostic markers for breast cancer |
title_sort | eukaryotic initiation factor 4e (eif4e) and angiogenesis: prognostic markers for breast cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1599748/ https://www.ncbi.nlm.nih.gov/pubmed/17010208 http://dx.doi.org/10.1186/1471-2407-6-231 |
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