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Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer
BACKGROUND: The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal canc...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1629022/ https://www.ncbi.nlm.nih.gov/pubmed/17054783 http://dx.doi.org/10.1186/1471-2407-6-250 |
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author | Solmi, Rossella Ugolini, Giampaolo Rosati, Giancarlo Zanotti, Simone Lauriola, Mattia Montroni, Isacco del Governatore, Marco Caira, Antonello Taffurelli, Mario Santini, Donatella Coppola, Domenico Guidotti, Lia Carinci, Paolo Strippoli, Pierluigi |
author_facet | Solmi, Rossella Ugolini, Giampaolo Rosati, Giancarlo Zanotti, Simone Lauriola, Mattia Montroni, Isacco del Governatore, Marco Caira, Antonello Taffurelli, Mario Santini, Donatella Coppola, Domenico Guidotti, Lia Carinci, Paolo Strippoli, Pierluigi |
author_sort | Solmi, Rossella |
collection | PubMed |
description | BACKGROUND: The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. METHODS: In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). RESULTS: Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. CONCLUSION: The design of new approaches to identify such markers is warranted. |
format | Text |
id | pubmed-1629022 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-16290222006-10-31 Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer Solmi, Rossella Ugolini, Giampaolo Rosati, Giancarlo Zanotti, Simone Lauriola, Mattia Montroni, Isacco del Governatore, Marco Caira, Antonello Taffurelli, Mario Santini, Donatella Coppola, Domenico Guidotti, Lia Carinci, Paolo Strippoli, Pierluigi BMC Cancer Research Article BACKGROUND: The efficacy of screening for colorectal cancer using a simple blood-based assay for the detection of tumor cells disseminated in the circulation at an early stage of the disease is gaining positive feedback from several lines of research. This method seems able to reduce colorectal cancer mortality and may replace colonoscopy as the most effective means of detecting colonic lesions. METHODS: In this work, we present a new microarray-based high-throughput screening method to identifying candidate marker mRNAs for the early detection of epithelial cells diluted in peripheral blood cells. This method includes 1. direct comparison of different samples of colonic mucosa and of blood cells to identify consistent epithelial-specific mRNAs from among 20,000 cDNA assayed by microarray slides; 2. identification of candidate marker mRNAs by data analysis, which allowed selection of only 10 putative differentially expressed genes; 3. Selection of some of the most suitable mRNAs (TMEM69, RANBP3 and PRSS22) that were assayed in blood samples from normal subjects and patients with colon cancer as possible markers for the presence of epithelial cells in the blood, using reverse transcription – polymerase chain reaction (RT-PCR). RESULTS: Our present results seem to provide an indication, for the first time obtained by genome-scale screening, that a suitable and consistent colon epithelium mRNA marker may be difficult to identify. CONCLUSION: The design of new approaches to identify such markers is warranted. BioMed Central 2006-10-20 /pmc/articles/PMC1629022/ /pubmed/17054783 http://dx.doi.org/10.1186/1471-2407-6-250 Text en Copyright © 2006 Solmi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Solmi, Rossella Ugolini, Giampaolo Rosati, Giancarlo Zanotti, Simone Lauriola, Mattia Montroni, Isacco del Governatore, Marco Caira, Antonello Taffurelli, Mario Santini, Donatella Coppola, Domenico Guidotti, Lia Carinci, Paolo Strippoli, Pierluigi Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer |
title | Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer |
title_full | Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer |
title_fullStr | Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer |
title_full_unstemmed | Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer |
title_short | Microarray-based identification and RT-PCR test screening for epithelial-specific mRNAs in peripheral blood of patients with colon cancer |
title_sort | microarray-based identification and rt-pcr test screening for epithelial-specific mrnas in peripheral blood of patients with colon cancer |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1629022/ https://www.ncbi.nlm.nih.gov/pubmed/17054783 http://dx.doi.org/10.1186/1471-2407-6-250 |
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