Cargando…
A real-time assay for monitoring nucleic acid cleavage by quadruplex formation
Direct and straightforward methods to follow nucleic acid cleavage are needed. A spectrophotometric quadruplex formation assay (QFA) was developed, which allows real-time monitoring of site-specific cleavage of nucleic acids. QFA was applied to study both protein and nucleic acid restriction enzymes...
Autor principal: | Kankia, Besik I. |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635264/ https://www.ncbi.nlm.nih.gov/pubmed/17071965 http://dx.doi.org/10.1093/nar/gkl594 |
Ejemplares similares
-
New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Förster resonance energy transfer probes in 5′-nuclease assays
por: Kutyavin, Igor V.
Publicado: (2010) -
Iso-FRET: an isothermal competition assay to analyze quadruplex formation in vitro
por: Luo, Yu, et al.
Publicado: (2022) -
Real-time investigation of nucleic acids phosphorylation process using molecular beacons
por: Tang, Zhiwen, et al.
Publicado: (2005) -
Quadruplex-and-Mg(2+) Connection (QMC) of DNA
por: Kankia, Besik
Publicado: (2015) -
Improved assay-dependent searching of nucleic acid sequence databases
por: Gans, Jason D., et al.
Publicado: (2008)