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Dynamic assembly of primers on nucleic acid templates

A strategy is presented that uses dynamic equlibria to assemble in situ composite DNA polymerase primers, having lengths of 14 or 16 nt, from DNA fragments that are 6 or 8 nt in length. In this implementation, the fragments are transiently joined under conditions of dynamic equilibrium by an imine l...

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Detalles Bibliográficos
Autores principales: Leal, Nicole A., Sukeda, Makoto, Benner, Steven A.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635275/
https://www.ncbi.nlm.nih.gov/pubmed/16963776
http://dx.doi.org/10.1093/nar/gkl625

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