Cargando…

Nanoliter high throughput quantitative PCR

Understanding biological complexity arising from patterns of gene expression requires accurate and precise measurement of RNA levels across large numbers of genes simultaneously. Real time PCR (RT-PCR) in a microtiter plate is the preferred method for quantitative transcriptional analysis but scalin...

Descripción completa

Detalles Bibliográficos
Autores principales:	Morrison, Tom, Hurley, James, Garcia, Javier, Yoder, Karl, Katz, Arrin, Roberts, Douglas, Cho, Jamie, Kanigan, Tanya, Ilyin, Sergey E., Horowitz, Daniel, Dixon, James M., Brenan, Colin J.H.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635282/ https://www.ncbi.nlm.nih.gov/pubmed/17000636 http://dx.doi.org/10.1093/nar/gkl639

Ejemplares similares

Novel method for high-throughput colony PCR screening in nanoliter-reactors
por: Walser, Marcel, et al.
Publicado: (2009)

Sources of PCR-induced distortions in high-throughput sequencing data sets
por: Kebschull, Justus M., et al.
Publicado: (2015)

From micrograms to picograms: quantitative PCR reduces the material demands of high-throughput sequencing
por: Meyer, Matthias, et al.
Publicado: (2008)

Long-range, high-throughput haplotype determination via haplotype-fusion PCR and ligation haplotyping
por: Turner, Daniel J., et al.
Publicado: (2008)

High-throughput stem-loop RT-qPCR miRNA expression profiling using minute amounts of input RNA
por: Mestdagh, Pieter, et al.
Publicado: (2008)

PCR amplification from single DNA molecules on magnetic beads in emulsion: application for high-throughput screening of transcription factor targets
por: Kojima, Takaaki, et al.
Publicado: (2005)

Optimization of experimental design parameters for high-throughput chromatin immunoprecipitation studies
por: Ponzielli, Romina, et al.
Publicado: (2008)

Sensitive Melting Analysis after Real Time- Methylation Specific PCR (SMART-MSP): high-throughput and probe-free quantitative DNA methylation detection
por: Kristensen, Lasse S., et al.
Publicado: (2008)

Characterization of L1 retrotransposition with high-throughput dual-luciferase assays
por: Xie, Yi, et al.
Publicado: (2011)

OUHP: an optimized universal hairpin primer system for cost-effective and high-throughput RT-qPCR-based quantification of microRNA (miRNA) expression
por: He, Fang, et al.
Publicado: (2021)

A high-throughput alpha particle irradiation system for monitoring DNA damage repair, genome instability and screening in human cell and yeast model systems
por: Stanley, Fintan K T, et al.
Publicado: (2020)

A method for counting PCR template molecules with application to next-generation sequencing
por: Casbon, James A., et al.
Publicado: (2011)

Analyzing editosome function in high-throughput
por: Del Campo, Cristian, et al.
Publicado: (2020)

SiteFinding-PCR: a simple and efficient PCR method for chromosome walking
por: Tan, Guihong, et al.
Publicado: (2005)

Reflex: intramolecular barcoding of long-range PCR products for sequencing multiple pooled DNAs
por: Casbon, James A., et al.
Publicado: (2013)

Comparison of microfluidic digital PCR and conventional quantitative PCR for measuring copy number variation
por: Whale, Alexandra S., et al.
Publicado: (2012)

High-throughput methods for measuring DNA thermodynamics
por: Bae, Jin H, et al.
Publicado: (2020)

Hot Start PCR with heat-activatable primers: a novel approach for improved PCR performance
por: Lebedev, Alexandre V., et al.
Publicado: (2008)

Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR
por: Lievens, Antoon, et al.
Publicado: (2012)

High-throughput assays for DNA gyrase and other topoisomerases
por: Maxwell, Anthony, et al.
Publicado: (2006)

High-throughput detection and multiplex identification of cell contaminations
por: Schmitt, Markus, et al.
Publicado: (2009)

Fluorescence strategies for high-throughput quantification of protein interactions
por: Hieb, Aaron R., et al.
Publicado: (2012)

A high-throughput approach to profile RNA structure
por: Delli Ponti, Riccardo, et al.
Publicado: (2017)

Phage display mediated immuno-PCR
por: Guo, Yong-Chao, et al.
Publicado: (2006)

Genomic DNA functions as a universal external standard in quantitative real-time PCR
por: Yun, James J., et al.
Publicado: (2006)

Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
por: Shaik, Gouse M., et al.
Publicado: (2008)

Accurate quantification of DNA using on-site PCR (osPCR) by characterizing DNA amplification at single-molecule resolution
por: Ding, Ruihua, et al.
Publicado: (2023)

Multiplex PCR: use of heat-stable Thermus thermophilus RecA protein to minimize non-specific PCR products
por: Shigemori, Yasushi, et al.
Publicado: (2005)

Targeted high-throughput sequencing of tagged nucleic acid samples
por: Meyer, Matthias, et al.
Publicado: (2007)

High-throughput discovery of rare human nucleotide polymorphisms by Ecotilling
por: Till, Bradley J., et al.
Publicado: (2006)

Quantifying selection in high-throughput Immunoglobulin sequencing data sets
por: Yaari, Gur, et al.
Publicado: (2012)

Summarizing and correcting the GC content bias in high-throughput sequencing
por: Benjamini, Yuval, et al.
Publicado: (2012)

Detecting Alu insertions from high-throughput sequencing data
por: David, Matei, et al.
Publicado: (2013)

High-throughput, cost-effective verification of structural DNA assembly
por: Dharmadi, Yandi, et al.
Publicado: (2014)

High-throughput assay and engineering of self-cleaving ribozymes by sequencing
por: Kobori, Shungo, et al.
Publicado: (2015)

Determination of tRNA aminoacylation levels by high-throughput sequencing
por: Evans, Molly E., et al.
Publicado: (2017)

Using high-throughput barcode sequencing to efficiently map connectomes
por: Peikon, Ian D., et al.
Publicado: (2017)

Predicting failure rate of PCR in large genomes
por: Andreson, Reidar, et al.
Publicado: (2008)

DNA analysis with multiplex microarray-enhanced PCR
por: Pemov, A., et al.
Publicado: (2005)

A thermodynamic approach to PCR primer design
por: Mann, Tobias, et al.
Publicado: (2009)

Cannot write session to /tmp/vufind_sessions/sess_36jtphnml82bpe692g23crej5t