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Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli

Reliable methods for conditional gene silencing in bacteria have been elusive. To improve silencing by expressed antisense RNAs (asRNAs), we systematically altered several design parameters and targeted multiple reporter and essential genes in Escherichia coli. A paired termini (PT) design, where fl...

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Detalles Bibliográficos
Autores principales:	Nakashima, Nobutaka, Tamura, Tomohiro, Good, Liam
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635301/ https://www.ncbi.nlm.nih.gov/pubmed/17062631 http://dx.doi.org/10.1093/nar/gkl697

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