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Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis

BACKGROUND: The nuclear lamina is a protein meshwork lining the inner nuclear membrane, which contains a polymer of nuclear lamins associated with transmembrane proteins of the inner nuclear membrane. The lamina is involved in nuclear structure, gene expression, and association of the cytoplasmic cy...

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Autores principales: Chen, I-Hsiung Brandon, Huber, Michael, Guan, Tinglu, Bubeck, Anja, Gerace, Larry
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635557/
https://www.ncbi.nlm.nih.gov/pubmed/17062158
http://dx.doi.org/10.1186/1471-2121-7-38
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author Chen, I-Hsiung Brandon
Huber, Michael
Guan, Tinglu
Bubeck, Anja
Gerace, Larry
author_facet Chen, I-Hsiung Brandon
Huber, Michael
Guan, Tinglu
Bubeck, Anja
Gerace, Larry
author_sort Chen, I-Hsiung Brandon
collection PubMed
description BACKGROUND: The nuclear lamina is a protein meshwork lining the inner nuclear membrane, which contains a polymer of nuclear lamins associated with transmembrane proteins of the inner nuclear membrane. The lamina is involved in nuclear structure, gene expression, and association of the cytoplasmic cytoskeleton with the nucleus. We previously identified a group of 67 novel putative nuclear envelope transmembrane proteins (NETs) in a large-scale proteomics analysis. Because mutations in lamina proteins have been linked to several human diseases affecting skeletal muscle, we examined NET expression during differentiation of C2C12 myoblasts. Our goal was to identify new nuclear envelope and lamina components whose expression is coordinated with muscle differentiation. RESULTS: Using transcriptional microarray analysis, we found that expression of 6 of the NETs significantly increases during myoblast differentiation. We confirmed these results using quantitative RT-PCR, and furthermore, found that all 6 NETs are expressed at high levels in adult mouse skeletal muscle relative to 9 other tissues examined. Using epitope-tagged cDNAs, we determined that the 5 NETs we could analyze (NETs 9, 25, 32, 37 and 39) all target to the nuclear envelope in C2C12 cells. Furthermore, the 3 NETs that we could analyze by immunoblotting were highly enriched in nuclear envelopes relative to microsomal membranes purified from mouse liver. Database searches showed that 4 of the 6 up-regulated NETs contain regions of homology to proteins previously linked to signaling. CONCLUSION: This work identified 6 NETs that are predicted to have important functions in muscle development and/or maintenance from their expression patterns during myoblast differentiation and in mouse tissues. We confirmed that 5 of these NETs are authentic nuclear envelope proteins. Four members of this group have potential signaling functions at the NE, based on their sequence homologies.
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spelling pubmed-16355572006-11-10 Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis Chen, I-Hsiung Brandon Huber, Michael Guan, Tinglu Bubeck, Anja Gerace, Larry BMC Cell Biol Research Article BACKGROUND: The nuclear lamina is a protein meshwork lining the inner nuclear membrane, which contains a polymer of nuclear lamins associated with transmembrane proteins of the inner nuclear membrane. The lamina is involved in nuclear structure, gene expression, and association of the cytoplasmic cytoskeleton with the nucleus. We previously identified a group of 67 novel putative nuclear envelope transmembrane proteins (NETs) in a large-scale proteomics analysis. Because mutations in lamina proteins have been linked to several human diseases affecting skeletal muscle, we examined NET expression during differentiation of C2C12 myoblasts. Our goal was to identify new nuclear envelope and lamina components whose expression is coordinated with muscle differentiation. RESULTS: Using transcriptional microarray analysis, we found that expression of 6 of the NETs significantly increases during myoblast differentiation. We confirmed these results using quantitative RT-PCR, and furthermore, found that all 6 NETs are expressed at high levels in adult mouse skeletal muscle relative to 9 other tissues examined. Using epitope-tagged cDNAs, we determined that the 5 NETs we could analyze (NETs 9, 25, 32, 37 and 39) all target to the nuclear envelope in C2C12 cells. Furthermore, the 3 NETs that we could analyze by immunoblotting were highly enriched in nuclear envelopes relative to microsomal membranes purified from mouse liver. Database searches showed that 4 of the 6 up-regulated NETs contain regions of homology to proteins previously linked to signaling. CONCLUSION: This work identified 6 NETs that are predicted to have important functions in muscle development and/or maintenance from their expression patterns during myoblast differentiation and in mouse tissues. We confirmed that 5 of these NETs are authentic nuclear envelope proteins. Four members of this group have potential signaling functions at the NE, based on their sequence homologies. BioMed Central 2006-10-24 /pmc/articles/PMC1635557/ /pubmed/17062158 http://dx.doi.org/10.1186/1471-2121-7-38 Text en Copyright © 2006 Chen et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Chen, I-Hsiung Brandon
Huber, Michael
Guan, Tinglu
Bubeck, Anja
Gerace, Larry
Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis
title Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis
title_full Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis
title_fullStr Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis
title_full_unstemmed Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis
title_short Nuclear envelope transmembrane proteins (NETs) that are up-regulated during myogenesis
title_sort nuclear envelope transmembrane proteins (nets) that are up-regulated during myogenesis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1635557/
https://www.ncbi.nlm.nih.gov/pubmed/17062158
http://dx.doi.org/10.1186/1471-2121-7-38
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AT bubeckanja nuclearenvelopetransmembraneproteinsnetsthatareupregulatedduringmyogenesis
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