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An improved method for preparing Agrobacterium cells that simplifies the Arabidopsis transformation protocol
BACKGROUND: The Agrobacterium vacuum (Bechtold et al 1993) and floral-dip (Clough and Bent 1998) are very efficient methods for generating transgenic Arabidopsis plants. These methods allow plant transformation without the need for tissue culture. Large volumes of bacterial cultures grown in liquid...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636042/ https://www.ncbi.nlm.nih.gov/pubmed/17062132 http://dx.doi.org/10.1186/1746-4811-2-16 |