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Direct and random routing of a molecular motor protein at a DNA junction
With the aim of investigating how motor proteins negotiate DNA nanostructures, we produced test circuits based on recombination intermediates in which 1D translocation across a Holliday junction (HJ) could be assessed by subsequent triplex displacement signals on each DNA arm. Using the EcoR124I res...
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636355/ https://www.ncbi.nlm.nih.gov/pubmed/16936313 http://dx.doi.org/10.1093/nar/gkl569 |
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author | Stanley, Louise K. Szczelkun, Mark D. |
author_facet | Stanley, Louise K. Szczelkun, Mark D. |
author_sort | Stanley, Louise K. |
collection | PubMed |
description | With the aim of investigating how motor proteins negotiate DNA nanostructures, we produced test circuits based on recombination intermediates in which 1D translocation across a Holliday junction (HJ) could be assessed by subsequent triplex displacement signals on each DNA arm. Using the EcoR124I restriction-modification enzyme, a 3′–5′ double-strand DNA (dsDNA) translocase, we could show that the motor will tend to follow its translocated strand across a junction. Nonetheless, as the frequency of junction bypass events increases, the motor will occasionally jump tracks. |
format | Text |
id | pubmed-1636355 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-16363552006-11-29 Direct and random routing of a molecular motor protein at a DNA junction Stanley, Louise K. Szczelkun, Mark D. Nucleic Acids Res Nucleic Acid Enzymes With the aim of investigating how motor proteins negotiate DNA nanostructures, we produced test circuits based on recombination intermediates in which 1D translocation across a Holliday junction (HJ) could be assessed by subsequent triplex displacement signals on each DNA arm. Using the EcoR124I restriction-modification enzyme, a 3′–5′ double-strand DNA (dsDNA) translocase, we could show that the motor will tend to follow its translocated strand across a junction. Nonetheless, as the frequency of junction bypass events increases, the motor will occasionally jump tracks. Oxford University Press 2006-09 2006-08-26 /pmc/articles/PMC1636355/ /pubmed/16936313 http://dx.doi.org/10.1093/nar/gkl569 Text en © 2006 The Author(s) |
spellingShingle | Nucleic Acid Enzymes Stanley, Louise K. Szczelkun, Mark D. Direct and random routing of a molecular motor protein at a DNA junction |
title | Direct and random routing of a molecular motor protein at a DNA junction |
title_full | Direct and random routing of a molecular motor protein at a DNA junction |
title_fullStr | Direct and random routing of a molecular motor protein at a DNA junction |
title_full_unstemmed | Direct and random routing of a molecular motor protein at a DNA junction |
title_short | Direct and random routing of a molecular motor protein at a DNA junction |
title_sort | direct and random routing of a molecular motor protein at a dna junction |
topic | Nucleic Acid Enzymes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636355/ https://www.ncbi.nlm.nih.gov/pubmed/16936313 http://dx.doi.org/10.1093/nar/gkl569 |
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