Cargando…

Direct and random routing of a molecular motor protein at a DNA junction

With the aim of investigating how motor proteins negotiate DNA nanostructures, we produced test circuits based on recombination intermediates in which 1D translocation across a Holliday junction (HJ) could be assessed by subsequent triplex displacement signals on each DNA arm. Using the EcoR124I res...

Descripción completa

Detalles Bibliográficos
Autores principales: Stanley, Louise K., Szczelkun, Mark D.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636355/
https://www.ncbi.nlm.nih.gov/pubmed/16936313
http://dx.doi.org/10.1093/nar/gkl569
_version_ 1782130737179262976
author Stanley, Louise K.
Szczelkun, Mark D.
author_facet Stanley, Louise K.
Szczelkun, Mark D.
author_sort Stanley, Louise K.
collection PubMed
description With the aim of investigating how motor proteins negotiate DNA nanostructures, we produced test circuits based on recombination intermediates in which 1D translocation across a Holliday junction (HJ) could be assessed by subsequent triplex displacement signals on each DNA arm. Using the EcoR124I restriction-modification enzyme, a 3′–5′ double-strand DNA (dsDNA) translocase, we could show that the motor will tend to follow its translocated strand across a junction. Nonetheless, as the frequency of junction bypass events increases, the motor will occasionally jump tracks.
format Text
id pubmed-1636355
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-16363552006-11-29 Direct and random routing of a molecular motor protein at a DNA junction Stanley, Louise K. Szczelkun, Mark D. Nucleic Acids Res Nucleic Acid Enzymes With the aim of investigating how motor proteins negotiate DNA nanostructures, we produced test circuits based on recombination intermediates in which 1D translocation across a Holliday junction (HJ) could be assessed by subsequent triplex displacement signals on each DNA arm. Using the EcoR124I restriction-modification enzyme, a 3′–5′ double-strand DNA (dsDNA) translocase, we could show that the motor will tend to follow its translocated strand across a junction. Nonetheless, as the frequency of junction bypass events increases, the motor will occasionally jump tracks. Oxford University Press 2006-09 2006-08-26 /pmc/articles/PMC1636355/ /pubmed/16936313 http://dx.doi.org/10.1093/nar/gkl569 Text en © 2006 The Author(s)
spellingShingle Nucleic Acid Enzymes
Stanley, Louise K.
Szczelkun, Mark D.
Direct and random routing of a molecular motor protein at a DNA junction
title Direct and random routing of a molecular motor protein at a DNA junction
title_full Direct and random routing of a molecular motor protein at a DNA junction
title_fullStr Direct and random routing of a molecular motor protein at a DNA junction
title_full_unstemmed Direct and random routing of a molecular motor protein at a DNA junction
title_short Direct and random routing of a molecular motor protein at a DNA junction
title_sort direct and random routing of a molecular motor protein at a dna junction
topic Nucleic Acid Enzymes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636355/
https://www.ncbi.nlm.nih.gov/pubmed/16936313
http://dx.doi.org/10.1093/nar/gkl569
work_keys_str_mv AT stanleylouisek directandrandomroutingofamolecularmotorproteinatadnajunction
AT szczelkunmarkd directandrandomroutingofamolecularmotorproteinatadnajunction