Cargando…

Identification of miRNA targets with stable isotope labeling by amino acids in cell culture

miRNAs are small noncoding RNAs that regulate gene expression. We have used stable isotope labeling by amino acids in cell culture (SILAC) to investigate the effect of miRNA-1 on the HeLa cell proteome. Expression of 12 out of 504 investigated proteins was repressed by miRNA-1 transfection. This rep...

Descripción completa

Detalles Bibliográficos
Autores principales:	Vinther, Jeppe, Hedegaard, Mads M., Gardner, Paul P., Andersen, Jens S., Arctander, Peter
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2006
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636363/ https://www.ncbi.nlm.nih.gov/pubmed/16945957 http://dx.doi.org/10.1093/nar/gkl590

Ejemplares similares

Massive parallel-sequencing-based hydroxyl radical probing of RNA accessibility
por: Kielpinski, Lukasz Jan, et al.
Publicado: (2014)

miRdentify: high stringency miRNA predictor identifies several novel animal miRNAs
por: Hansen, Thomas B., et al.
Publicado: (2014)

Site-specific isotope labeling of long RNA for structural and mechanistic studies
por: Kawahara, Ikumi, et al.
Publicado: (2012)

miRNA target enrichment analysis reveals directly active miRNAs in health and disease
por: Steinfeld, Israel, et al.
Publicado: (2013)

Polysome shift assay for direct measurement of miRNA inhibition by anti-miRNA drugs
por: Androsavich, John R., et al.
Publicado: (2016)

Multiple segmental and selective isotope labeling of large RNA for NMR structural studies
por: Nelissen, Frank H. T., et al.
Publicado: (2008)

Impact of probe annotation on the integration of miRNA–mRNA expression profiles for miRNA target detection
por: Sales, Gabriele, et al.
Publicado: (2010)

Wiring miRNAs to pathways: a topological approach to integrate miRNA and mRNA expression profiles
por: Calura, Enrica, et al.
Publicado: (2014)

Detection of internal N7-methylguanosine (m(7)G) RNA modifications by mutational profiling sequencing
por: Enroth, Christel, et al.
Publicado: (2019)

miSTAR: miRNA target prediction through modeling quantitative and qualitative miRNA binding site information in a stacked model structure
por: Van Peer, Gert, et al.
Publicado: (2017)

miRVaS: a tool to predict the impact of genetic variants on miRNAs
por: Cammaerts, Sophia, et al.
Publicado: (2016)

Integrative analysis of gene and miRNA expression profiles with transcription factor–miRNA feed-forward loops identifies regulators in human cancers
por: Yan, Zhenyu, et al.
Publicado: (2012)

ncOrtho: efficient and reliable identification of miRNA orthologs
por: Langschied, Felix, et al.
Publicado: (2023)

miRspring: a compact standalone research tool for analyzing miRNA-seq data
por: Humphreys, David T., et al.
Publicado: (2013)

Chemo-enzymatic synthesis of site-specific isotopically labeled nucleotides for use in NMR resonance assignment, dynamics and structural characterizations
por: Longhini, Andrew P., et al.
Publicado: (2016)

Design and implementation of a synthetic pre-miR switch for controlling miRNA biogenesis in mammals
por: Atanasov, Janina, et al.
Publicado: (2017)

Lentivirus-mediated antagomir expression for specific inhibition of miRNA function
por: Scherr, Michaela, et al.
Publicado: (2007)

Prioritizing and selecting likely novel miRNAs from NGS data
por: Backes, Christina, et al.
Publicado: (2016)

Multiplexed miRNA northern blots via hybridization chain reaction
por: Schwarzkopf, Maayan, et al.
Publicado: (2016)

sPARTA: a parallelized pipeline for integrated analysis of plant miRNA and cleaved mRNA data sets, including new miRNA target-identification software
por: Kakrana, Atul, et al.
Publicado: (2014)

Rapid identification of regulatory microRNAs by miTRAP (miRNA trapping by RNA in vitro affinity purification)
por: Braun, Juliane, et al.
Publicado: (2014)

A fast, efficient and sequence-independent method for flexible multiple segmental isotope labeling of RNA using ribozyme and RNase H cleavage
por: Duss, Olivier, et al.
Publicado: (2010)

Combining asymmetric (13)C-labeling and isotopic filter/edit NOESY: a novel strategy for rapid and logical RNA resonance assignment
por: LeBlanc, Regan M., et al.
Publicado: (2017)

A fast ab-initio method for predicting miRNA precursors in genomes
por: Tempel, Sébastien, et al.
Publicado: (2012)

Tandem affinity purification of miRNA target mRNAs (TAP-Tar)
por: Nonne, Nora, et al.
Publicado: (2010)

Sensitive and specific miRNA detection method using SplintR Ligase
por: Jin, Jingmin, et al.
Publicado: (2016)

Identification of active miRNA promoters from nuclear run-on RNA sequencing
por: Liu, Qi, et al.
Publicado: (2017)

Gene-specific cell labeling using MiMIC transposons
por: Gnerer, Joshua P., et al.
Publicado: (2015)

A high throughput experimental approach to identify miRNA targets in human cells
por: Tan, Lu Ping, et al.
Publicado: (2009)

Heterogeneous ensemble approach with discriminative features and modified-SMOTEbagging for pre-miRNA classification
por: Lertampaiporn, Supatcha, et al.
Publicado: (2013)

3′LIFE: a functional assay to detect miRNA targets in high-throughput
por: Wolter, Justin M., et al.
Publicado: (2014)

RILES, a novel method for temporal analysis of the in vivo regulation of miRNA expression
por: Ezzine, Safia, et al.
Publicado: (2013)

Quantitative analysis of conditional gene inactivation using rationally designed, tetracycline-controlled miRNAs
por: Berger, Stefan M., et al.
Publicado: (2010)

Inferring probabilistic miRNA–mRNA interaction signatures in cancers: a role-switch approach
por: Li, Yue, et al.
Publicado: (2014)

Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification
por: Androvic, Peter, et al.
Publicado: (2017)

A UTF1-based selection system for stable homogeneously pluripotent human embryonic stem cell cultures
por: Tan, Shen Mynn, et al.
Publicado: (2007)

Discovery and visualization of miRNA–mRNA functional modules within integrated data using bicluster analysis
por: Bryan, Kenneth, et al.
Publicado: (2014)

TALEN/CRISPR-mediated engineering of a promoterless anti-viral RNAi hairpin into an endogenous miRNA locus
por: Senís, Elena, et al.
Publicado: (2017)

Identify bilayer modules via pseudo-3D clustering: applications to miRNA-gene bilayer networks
por: Xu, Yungang, et al.
Publicado: (2016)

Stable Isotope Labeling with Amino acids in Nematodes
por: Larance, Mark, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_6v2m9p53oas0nf4ms4h5vs8g52