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Tapping diversity lost in transformations—in vitro amplification of ligation reactions
Molecular evolution is a powerful means of engineering proteins. It usually requires the generation of a large recombinant DNA library of variants for cloning into a phage or plasmid vector, and the transformation of a host organism for expression and screening of the variant proteins. However, libr...
| Autores principales: | , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2006
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636367/ https://www.ncbi.nlm.nih.gov/pubmed/16945952 http://dx.doi.org/10.1093/nar/gkl605 |