Cargando…
A novel endonuclease IV post-PCR genotyping system
Here we describe a novel endonuclease IV (Endo IV) based assay utilizing a substrate that mimics the abasic lesions that normally occur in double-stranded DNA. The three component substrate is characterized by single-stranded DNA target, an oligonucleotide probe, separated from a helper oligonucleot...
Autores principales: | Kutyavin, Igor V., Milesi, Dave, Belousov, Yevgeniy, Podyminogin, Mikhail, Vorobiev, Alexei, Gorn, Vladimir, Lukhtanov, Eugeny A., Vermeulen, Nicolaas M. J., Mahoney, Walt |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2006
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1636472/ https://www.ncbi.nlm.nih.gov/pubmed/17012270 http://dx.doi.org/10.1093/nar/gkl679 |
Ejemplares similares
-
Novel DNA probes with low background and high hybridization-triggered fluorescence
por: Lukhtanov, Eugeny A., et al.
Publicado: (2007) -
New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Förster resonance energy transfer probes in 5′-nuclease assays
por: Kutyavin, Igor V.
Publicado: (2010) -
Use of extremely short Förster resonance energy transfer probes in real-time polymerase chain reaction
por: Kutyavin, Igor V.
Publicado: (2013) -
Preparation of selective and segmentally labeled single-stranded DNA for NMR by self-primed PCR and asymmetrical endonuclease double digestion
por: Nelissen, Frank H. T., et al.
Publicado: (2009) -
Massively parallel characterization of restriction endonucleases
por: Kamps-Hughes, Nick, et al.
Publicado: (2013)