Effect of polybrominated biphenyls on adenylate cyclase activity in rat lung alveoli.

The in vitro effect of polybrominated biphenyls (FireMaster BP-6) on adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] activity in plasma membranes of rat lung alveoli was determined. Two fractions of plasma membranes, PM-I (approximate d = 1.13 g/cm3) and PM-II (approximate d = 1....

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Detalles Bibliográficos
Autores principales: Sidhu, K S, Michelakis, A M
Formato: Texto
Lenguaje:English
Publicado: 1978
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1637478/
https://www.ncbi.nlm.nih.gov/pubmed/209995
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author Sidhu, K S
Michelakis, A M
author_facet Sidhu, K S
Michelakis, A M
author_sort Sidhu, K S
collection PubMed
description The in vitro effect of polybrominated biphenyls (FireMaster BP-6) on adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] activity in plasma membranes of rat lung alveoli was determined. Two fractions of plasma membranes, PM-I (approximate d = 1.13 g/cm3) and PM-II (approximate d = 1.16 g/cm3) were isolated under hypotonic homogenization and fractionation conditions (1mM NaHCO3, 5mM dithiothreitol, pH 7.5) by using differential centrifugation and nonlinear sucrose gradient centrifugation techniques. Polybrominated biphenyls (10 microgram/ml) dissolved in ethyl acetate (0.25%) stimulated basal adenylate cyclase activity of plasma membranes by 2.1 to 2.5-fold. The implication of this observation is discussed.
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spelling pubmed-16374782006-11-17 Effect of polybrominated biphenyls on adenylate cyclase activity in rat lung alveoli. Sidhu, K S Michelakis, A M Environ Health Perspect Research Article The in vitro effect of polybrominated biphenyls (FireMaster BP-6) on adenylate cyclase [ATP pyrophosphate lyase (cyclizing), EC 4.6.1.1] activity in plasma membranes of rat lung alveoli was determined. Two fractions of plasma membranes, PM-I (approximate d = 1.13 g/cm3) and PM-II (approximate d = 1.16 g/cm3) were isolated under hypotonic homogenization and fractionation conditions (1mM NaHCO3, 5mM dithiothreitol, pH 7.5) by using differential centrifugation and nonlinear sucrose gradient centrifugation techniques. Polybrominated biphenyls (10 microgram/ml) dissolved in ethyl acetate (0.25%) stimulated basal adenylate cyclase activity of plasma membranes by 2.1 to 2.5-fold. The implication of this observation is discussed. 1978-04 /pmc/articles/PMC1637478/ /pubmed/209995 Text en
spellingShingle Research Article
Sidhu, K S
Michelakis, A M
Effect of polybrominated biphenyls on adenylate cyclase activity in rat lung alveoli.
title Effect of polybrominated biphenyls on adenylate cyclase activity in rat lung alveoli.
title_full Effect of polybrominated biphenyls on adenylate cyclase activity in rat lung alveoli.
title_fullStr Effect of polybrominated biphenyls on adenylate cyclase activity in rat lung alveoli.
title_full_unstemmed Effect of polybrominated biphenyls on adenylate cyclase activity in rat lung alveoli.
title_short Effect of polybrominated biphenyls on adenylate cyclase activity in rat lung alveoli.
title_sort effect of polybrominated biphenyls on adenylate cyclase activity in rat lung alveoli.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1637478/
https://www.ncbi.nlm.nih.gov/pubmed/209995
work_keys_str_mv AT sidhuks effectofpolybrominatedbiphenylsonadenylatecyclaseactivityinratlungalveoli
AT michelakisam effectofpolybrominatedbiphenylsonadenylatecyclaseactivityinratlungalveoli

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