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Effects of bovine spermatozoa preparation on embryonic development in vitro

The aim of our research was to examine the ability of density gradient preparation BoviPure(® )and swim up method on bull sperm separation and in vitro embryo production (IVP) systems. Frozen/thawed semen from six Simmental bulls was pooled and treated using both methods. The sperm motility, concent...

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Autores principales: Samardzija, Marko, Karadjole, Martina, Getz, Iva, Makek, Zdenko, Cergolj, Marijan, Dobranic, Tomislav
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1647281/
https://www.ncbi.nlm.nih.gov/pubmed/17101040
http://dx.doi.org/10.1186/1477-7827-4-58
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author Samardzija, Marko
Karadjole, Martina
Getz, Iva
Makek, Zdenko
Cergolj, Marijan
Dobranic, Tomislav
author_facet Samardzija, Marko
Karadjole, Martina
Getz, Iva
Makek, Zdenko
Cergolj, Marijan
Dobranic, Tomislav
author_sort Samardzija, Marko
collection PubMed
description The aim of our research was to examine the ability of density gradient preparation BoviPure(® )and swim up method on bull sperm separation and in vitro embryo production (IVP) systems. Frozen/thawed semen from six Simmental bulls was pooled and treated using both methods. The sperm motility, concentration, membrane activity, membrane integrity and acrosomal status were evaluated and compared before and after sperm processing using BoviPure(® )and swim up methods. We also evaluated and compared cleavage rates, embryo yield and quality between the methods. There were significant differences (P < 0.05) between the sperm characteristics before and after BoviPure(®), but not after swim up method. However, there were significant differences for sperm results among those two mentioned methods. A total of 641 oocytes were matured and fertilized in vitro and cultured in SOFaaBSA. The percentage of cleavage (Day 2) and the percentage of hatched embryos (Day 9) were similar for both methods. However, embryo production rate (Day 7) was significantly higher using BoviPure(® )method (P < 0.05). Also, total cell number and embryo differential staining (inner cell mass and trophectoderm cells) of Day 7 morulas and blastocysts showed that BoviPure(® )treated sperm displayed higher quality embryos compared to swim up method (P < 0.05). Our results indicate that BoviPure(® )method has an enhanced capacity in sperm selection for in vitro embryo production when compared with swim up method. So, we concluded that BoviPure(® )could be considered as a better alternative to swim up method for separating bull spermatozoa from frozen/thawed semen for IVP of bovine embryos.
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spelling pubmed-16472812006-11-18 Effects of bovine spermatozoa preparation on embryonic development in vitro Samardzija, Marko Karadjole, Martina Getz, Iva Makek, Zdenko Cergolj, Marijan Dobranic, Tomislav Reprod Biol Endocrinol Research The aim of our research was to examine the ability of density gradient preparation BoviPure(® )and swim up method on bull sperm separation and in vitro embryo production (IVP) systems. Frozen/thawed semen from six Simmental bulls was pooled and treated using both methods. The sperm motility, concentration, membrane activity, membrane integrity and acrosomal status were evaluated and compared before and after sperm processing using BoviPure(® )and swim up methods. We also evaluated and compared cleavage rates, embryo yield and quality between the methods. There were significant differences (P < 0.05) between the sperm characteristics before and after BoviPure(®), but not after swim up method. However, there were significant differences for sperm results among those two mentioned methods. A total of 641 oocytes were matured and fertilized in vitro and cultured in SOFaaBSA. The percentage of cleavage (Day 2) and the percentage of hatched embryos (Day 9) were similar for both methods. However, embryo production rate (Day 7) was significantly higher using BoviPure(® )method (P < 0.05). Also, total cell number and embryo differential staining (inner cell mass and trophectoderm cells) of Day 7 morulas and blastocysts showed that BoviPure(® )treated sperm displayed higher quality embryos compared to swim up method (P < 0.05). Our results indicate that BoviPure(® )method has an enhanced capacity in sperm selection for in vitro embryo production when compared with swim up method. So, we concluded that BoviPure(® )could be considered as a better alternative to swim up method for separating bull spermatozoa from frozen/thawed semen for IVP of bovine embryos. BioMed Central 2006-11-13 /pmc/articles/PMC1647281/ /pubmed/17101040 http://dx.doi.org/10.1186/1477-7827-4-58 Text en Copyright © 2006 Samardzija et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Samardzija, Marko
Karadjole, Martina
Getz, Iva
Makek, Zdenko
Cergolj, Marijan
Dobranic, Tomislav
Effects of bovine spermatozoa preparation on embryonic development in vitro
title Effects of bovine spermatozoa preparation on embryonic development in vitro
title_full Effects of bovine spermatozoa preparation on embryonic development in vitro
title_fullStr Effects of bovine spermatozoa preparation on embryonic development in vitro
title_full_unstemmed Effects of bovine spermatozoa preparation on embryonic development in vitro
title_short Effects of bovine spermatozoa preparation on embryonic development in vitro
title_sort effects of bovine spermatozoa preparation on embryonic development in vitro
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1647281/
https://www.ncbi.nlm.nih.gov/pubmed/17101040
http://dx.doi.org/10.1186/1477-7827-4-58
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