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An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development

BACKGROUND: Accuracy in quantitative real-time RT-PCR is dependent on high quality RNA, consistent cDNA synthesis, and validated stable reference genes for data normalization. Reference genes used for normalization impact the results generated from expression studies and, hence, should be evaluated...

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Detalles Bibliográficos
Autores principales:	Reid, Karen E, Olsson, Niclas, Schlosser, James, Peng, Fred, Lund, Steven T
Formato:	Texto
Lenguaje:	English
Publicado:	BioMed Central 2006
Materias:	Methodology Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1654153/ https://www.ncbi.nlm.nih.gov/pubmed/17105665 http://dx.doi.org/10.1186/1471-2229-6-27

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