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Functional interaction between RNase III and the Escherichia coli ribosome
BACKGROUND: RNase III is a dsRNA specific endoribonuclease which is involved in the primary processing of rRNA and several mRNA species in bacteria. Both primary structural elements and the secondary structure of the substrate RNA play a role in cleavage specificity. RESULTS: We have analyzed RNase...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2003
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC165447/ https://www.ncbi.nlm.nih.gov/pubmed/12814522 http://dx.doi.org/10.1186/1471-2199-4-8 |
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author | Allas, Ülar Liiv, Aivar Remme, Jaanus |
author_facet | Allas, Ülar Liiv, Aivar Remme, Jaanus |
author_sort | Allas, Ülar |
collection | PubMed |
description | BACKGROUND: RNase III is a dsRNA specific endoribonuclease which is involved in the primary processing of rRNA and several mRNA species in bacteria. Both primary structural elements and the secondary structure of the substrate RNA play a role in cleavage specificity. RESULTS: We have analyzed RNase III cleavage sites around both ends of pre-23 S rRNA in the ribosome and in the protein-free pre-rRNA. It was found that in the protein-free pre-23 S rRNA the main cleavage site is at position (-7) in respect of the mature 5' end. When pre-23 S rRNA was in 70 S ribosomes or in 50 S subunits, the RNase III cleavage occurred at position (-3). We have demonstrated that RNase III interacts with both ribosomal subunits and with even higher affinity with 70 S ribosomes. Association of RNase III with 70 S ribosomes cannot be dissociated by poly(U) RNA indicating that the binding is specific. CONCLUSIONS: In addition to the primary and secondary structural elements in RNA, protein binding to substrate RNA can be a determinant of the RNase III cleavage site. |
format | Text |
id | pubmed-165447 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2003 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-1654472003-07-16 Functional interaction between RNase III and the Escherichia coli ribosome Allas, Ülar Liiv, Aivar Remme, Jaanus BMC Mol Biol Research Article BACKGROUND: RNase III is a dsRNA specific endoribonuclease which is involved in the primary processing of rRNA and several mRNA species in bacteria. Both primary structural elements and the secondary structure of the substrate RNA play a role in cleavage specificity. RESULTS: We have analyzed RNase III cleavage sites around both ends of pre-23 S rRNA in the ribosome and in the protein-free pre-rRNA. It was found that in the protein-free pre-23 S rRNA the main cleavage site is at position (-7) in respect of the mature 5' end. When pre-23 S rRNA was in 70 S ribosomes or in 50 S subunits, the RNase III cleavage occurred at position (-3). We have demonstrated that RNase III interacts with both ribosomal subunits and with even higher affinity with 70 S ribosomes. Association of RNase III with 70 S ribosomes cannot be dissociated by poly(U) RNA indicating that the binding is specific. CONCLUSIONS: In addition to the primary and secondary structural elements in RNA, protein binding to substrate RNA can be a determinant of the RNase III cleavage site. BioMed Central 2003-06-18 /pmc/articles/PMC165447/ /pubmed/12814522 http://dx.doi.org/10.1186/1471-2199-4-8 Text en Copyright © 2003 Allas et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL. |
spellingShingle | Research Article Allas, Ülar Liiv, Aivar Remme, Jaanus Functional interaction between RNase III and the Escherichia coli ribosome |
title | Functional interaction between RNase III and the Escherichia coli ribosome |
title_full | Functional interaction between RNase III and the Escherichia coli ribosome |
title_fullStr | Functional interaction between RNase III and the Escherichia coli ribosome |
title_full_unstemmed | Functional interaction between RNase III and the Escherichia coli ribosome |
title_short | Functional interaction between RNase III and the Escherichia coli ribosome |
title_sort | functional interaction between rnase iii and the escherichia coli ribosome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC165447/ https://www.ncbi.nlm.nih.gov/pubmed/12814522 http://dx.doi.org/10.1186/1471-2199-4-8 |
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