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Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination
BACKGROUND: The nosocomial acquisition of Candida albicans is a growing concern in intensive care units (ICUs) and understanding the route of contamination is relevant for infection control guidelines. METHODS: To analyze whether there is a specific ecology for any given hospital, we genotyped C. al...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1660576/ https://www.ncbi.nlm.nih.gov/pubmed/17101036 http://dx.doi.org/10.1186/1471-2334-6-162 |
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author | Eloy, Odile Marque, Stéphanie Botterel, Françoise Stephan, François Costa, Jean-Marc Lasserre, Virginie Bretagne, Stéphane |
author_facet | Eloy, Odile Marque, Stéphanie Botterel, Françoise Stephan, François Costa, Jean-Marc Lasserre, Virginie Bretagne, Stéphane |
author_sort | Eloy, Odile |
collection | PubMed |
description | BACKGROUND: The nosocomial acquisition of Candida albicans is a growing concern in intensive care units (ICUs) and understanding the route of contamination is relevant for infection control guidelines. METHODS: To analyze whether there is a specific ecology for any given hospital, we genotyped C. albicans isolates of the ICU of Versailles hospital (Hospital A) and compared the results with those previously obtained in another ICU in Henri Mondor hospital (Hospital B) using three polymorphic microsatellite markers (PMM). RESULTS: Among 36 patients with at least one positive culture for C. albicans, 26 had a specific multilocus genotype, two shared a common multilocus genotype, and 8 had the most common multilocus genotype found in the general population. The time interval between periods of hospitalization between patients with common genotypes differed by 13 to 78 days, thus supporting a lack of direct contamination. To confirm this hypothesis, the multilocus genotypic distributions of the three PMM were compared between the two hospitals. No statistically significant difference was observed. Multiple correspondences analysis did not indicate the association of a multilocus genotypic distribution with any given hospital. CONCLUSION: The present epidemiological study supports the conclusions that each patient harbours his/her own isolate, and that nosocomial transmission is not common in any given ICU. This study also supports the usefulness and practicability of PMM for studying the epidemiology of C. albicans. |
format | Text |
id | pubmed-1660576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-16605762006-11-24 Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination Eloy, Odile Marque, Stéphanie Botterel, Françoise Stephan, François Costa, Jean-Marc Lasserre, Virginie Bretagne, Stéphane BMC Infect Dis Research Article BACKGROUND: The nosocomial acquisition of Candida albicans is a growing concern in intensive care units (ICUs) and understanding the route of contamination is relevant for infection control guidelines. METHODS: To analyze whether there is a specific ecology for any given hospital, we genotyped C. albicans isolates of the ICU of Versailles hospital (Hospital A) and compared the results with those previously obtained in another ICU in Henri Mondor hospital (Hospital B) using three polymorphic microsatellite markers (PMM). RESULTS: Among 36 patients with at least one positive culture for C. albicans, 26 had a specific multilocus genotype, two shared a common multilocus genotype, and 8 had the most common multilocus genotype found in the general population. The time interval between periods of hospitalization between patients with common genotypes differed by 13 to 78 days, thus supporting a lack of direct contamination. To confirm this hypothesis, the multilocus genotypic distributions of the three PMM were compared between the two hospitals. No statistically significant difference was observed. Multiple correspondences analysis did not indicate the association of a multilocus genotypic distribution with any given hospital. CONCLUSION: The present epidemiological study supports the conclusions that each patient harbours his/her own isolate, and that nosocomial transmission is not common in any given ICU. This study also supports the usefulness and practicability of PMM for studying the epidemiology of C. albicans. BioMed Central 2006-11-13 /pmc/articles/PMC1660576/ /pubmed/17101036 http://dx.doi.org/10.1186/1471-2334-6-162 Text en Copyright © 2006 Eloy et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Eloy, Odile Marque, Stéphanie Botterel, Françoise Stephan, François Costa, Jean-Marc Lasserre, Virginie Bretagne, Stéphane Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination |
title | Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination |
title_full | Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination |
title_fullStr | Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination |
title_full_unstemmed | Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination |
title_short | Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination |
title_sort | uniform distribution of three candida albicans microsatellite markers in two french icu populations supports a lack of nosocomial cross-contamination |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1660576/ https://www.ncbi.nlm.nih.gov/pubmed/17101036 http://dx.doi.org/10.1186/1471-2334-6-162 |
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