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Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination

BACKGROUND: The nosocomial acquisition of Candida albicans is a growing concern in intensive care units (ICUs) and understanding the route of contamination is relevant for infection control guidelines. METHODS: To analyze whether there is a specific ecology for any given hospital, we genotyped C. al...

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Autores principales: Eloy, Odile, Marque, Stéphanie, Botterel, Françoise, Stephan, François, Costa, Jean-Marc, Lasserre, Virginie, Bretagne, Stéphane
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1660576/
https://www.ncbi.nlm.nih.gov/pubmed/17101036
http://dx.doi.org/10.1186/1471-2334-6-162
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author Eloy, Odile
Marque, Stéphanie
Botterel, Françoise
Stephan, François
Costa, Jean-Marc
Lasserre, Virginie
Bretagne, Stéphane
author_facet Eloy, Odile
Marque, Stéphanie
Botterel, Françoise
Stephan, François
Costa, Jean-Marc
Lasserre, Virginie
Bretagne, Stéphane
author_sort Eloy, Odile
collection PubMed
description BACKGROUND: The nosocomial acquisition of Candida albicans is a growing concern in intensive care units (ICUs) and understanding the route of contamination is relevant for infection control guidelines. METHODS: To analyze whether there is a specific ecology for any given hospital, we genotyped C. albicans isolates of the ICU of Versailles hospital (Hospital A) and compared the results with those previously obtained in another ICU in Henri Mondor hospital (Hospital B) using three polymorphic microsatellite markers (PMM). RESULTS: Among 36 patients with at least one positive culture for C. albicans, 26 had a specific multilocus genotype, two shared a common multilocus genotype, and 8 had the most common multilocus genotype found in the general population. The time interval between periods of hospitalization between patients with common genotypes differed by 13 to 78 days, thus supporting a lack of direct contamination. To confirm this hypothesis, the multilocus genotypic distributions of the three PMM were compared between the two hospitals. No statistically significant difference was observed. Multiple correspondences analysis did not indicate the association of a multilocus genotypic distribution with any given hospital. CONCLUSION: The present epidemiological study supports the conclusions that each patient harbours his/her own isolate, and that nosocomial transmission is not common in any given ICU. This study also supports the usefulness and practicability of PMM for studying the epidemiology of C. albicans.
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spelling pubmed-16605762006-11-24 Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination Eloy, Odile Marque, Stéphanie Botterel, Françoise Stephan, François Costa, Jean-Marc Lasserre, Virginie Bretagne, Stéphane BMC Infect Dis Research Article BACKGROUND: The nosocomial acquisition of Candida albicans is a growing concern in intensive care units (ICUs) and understanding the route of contamination is relevant for infection control guidelines. METHODS: To analyze whether there is a specific ecology for any given hospital, we genotyped C. albicans isolates of the ICU of Versailles hospital (Hospital A) and compared the results with those previously obtained in another ICU in Henri Mondor hospital (Hospital B) using three polymorphic microsatellite markers (PMM). RESULTS: Among 36 patients with at least one positive culture for C. albicans, 26 had a specific multilocus genotype, two shared a common multilocus genotype, and 8 had the most common multilocus genotype found in the general population. The time interval between periods of hospitalization between patients with common genotypes differed by 13 to 78 days, thus supporting a lack of direct contamination. To confirm this hypothesis, the multilocus genotypic distributions of the three PMM were compared between the two hospitals. No statistically significant difference was observed. Multiple correspondences analysis did not indicate the association of a multilocus genotypic distribution with any given hospital. CONCLUSION: The present epidemiological study supports the conclusions that each patient harbours his/her own isolate, and that nosocomial transmission is not common in any given ICU. This study also supports the usefulness and practicability of PMM for studying the epidemiology of C. albicans. BioMed Central 2006-11-13 /pmc/articles/PMC1660576/ /pubmed/17101036 http://dx.doi.org/10.1186/1471-2334-6-162 Text en Copyright © 2006 Eloy et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Eloy, Odile
Marque, Stéphanie
Botterel, Françoise
Stephan, François
Costa, Jean-Marc
Lasserre, Virginie
Bretagne, Stéphane
Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination
title Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination
title_full Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination
title_fullStr Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination
title_full_unstemmed Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination
title_short Uniform distribution of three Candida albicans microsatellite markers in two French ICU populations supports a lack of nosocomial cross-contamination
title_sort uniform distribution of three candida albicans microsatellite markers in two french icu populations supports a lack of nosocomial cross-contamination
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1660576/
https://www.ncbi.nlm.nih.gov/pubmed/17101036
http://dx.doi.org/10.1186/1471-2334-6-162
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