Comparison of Plasmodium falciparum transfection methods

The development of an electroporation based transfection method for Plasmodium falciparum has been very successful for the study of some genes but its efficiency remains very low. While alternative approaches have been documented, electroporation of infected red blood cells generally remains the met...

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Autores principales: Skinner-Adams, Tina S, Lawrie, Paula M, Hawthorne, Paula L, Gardiner, Donald L, Trenholme, Katharine R
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2003
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC166142/
https://www.ncbi.nlm.nih.gov/pubmed/12869208
http://dx.doi.org/10.1186/1475-2875-2-19
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author Skinner-Adams, Tina S
Lawrie, Paula M
Hawthorne, Paula L
Gardiner, Donald L
Trenholme, Katharine R
author_facet Skinner-Adams, Tina S
Lawrie, Paula M
Hawthorne, Paula L
Gardiner, Donald L
Trenholme, Katharine R
author_sort Skinner-Adams, Tina S
collection PubMed
description The development of an electroporation based transfection method for Plasmodium falciparum has been very successful for the study of some genes but its efficiency remains very low. While alternative approaches have been documented, electroporation of infected red blood cells generally remains the method of choice for introducing DNA into P. falciparum. In this paper we compare four published transfection techniques in their ability to achieve stable transfections.
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spelling pubmed-1661422003-07-26 Comparison of Plasmodium falciparum transfection methods Skinner-Adams, Tina S Lawrie, Paula M Hawthorne, Paula L Gardiner, Donald L Trenholme, Katharine R Malar J Methodology The development of an electroporation based transfection method for Plasmodium falciparum has been very successful for the study of some genes but its efficiency remains very low. While alternative approaches have been documented, electroporation of infected red blood cells generally remains the method of choice for introducing DNA into P. falciparum. In this paper we compare four published transfection techniques in their ability to achieve stable transfections. BioMed Central 2003-06-27 /pmc/articles/PMC166142/ /pubmed/12869208 http://dx.doi.org/10.1186/1475-2875-2-19 Text en Copyright © 2003 Skinner-Adams et al; licensee BioMed Central Ltd. This is an Open Access article: verbatim copying and redistribution of this article are permitted in all media for any purpose, provided this notice is preserved along with the article's original URL.
spellingShingle Methodology
Skinner-Adams, Tina S
Lawrie, Paula M
Hawthorne, Paula L
Gardiner, Donald L
Trenholme, Katharine R
Comparison of Plasmodium falciparum transfection methods
title Comparison of Plasmodium falciparum transfection methods
title_full Comparison of Plasmodium falciparum transfection methods
title_fullStr Comparison of Plasmodium falciparum transfection methods
title_full_unstemmed Comparison of Plasmodium falciparum transfection methods
title_short Comparison of Plasmodium falciparum transfection methods
title_sort comparison of plasmodium falciparum transfection methods
topic Methodology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC166142/
https://www.ncbi.nlm.nih.gov/pubmed/12869208
http://dx.doi.org/10.1186/1475-2875-2-19
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